Sample Preparation and Post-separation Analysis

P0A6F9:

ID   CH10_ECOLI; STANDARD; 2DG.
AC   P0A6F9; P05380;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 16.
DE   RecName: Full=10 kDa chaperonin; AltName: Full=GroES protein; AltName:
DE   Full=Protein Cpn10;.
GN   Name=groS; Synonyms=groES, mopB; OrderedLocusNames=b4142, JW4102;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
RA   Gage D.J., Neidhardt F.C.;
RT   ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT   phosphorylation 2,4-dinitrophenol'';'';'';
RL   J. Bacteriol. 175(1):7105-7108(1993).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=87083376; PubMed=3539918; [NCBI, Expasy, EBI, Israel, Japan]
RA   Vanbogelen R.A., Kelley P.M., Neidhardt F.C.;
RT   ''''''Differential induction of heat shock, SOS, and oxidation stress
RT   regulons and accumulation of nucleotides in Escherichia coli'';'';'';
RL   J. Bacteriol. 169(1):26-32(1987).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [6]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: OLIGOMER OF 6 TO 8 SUBUNITS ARRANGED IN A RING 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000LQL=5.15/15660;
2D   -!-   EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY
2D         TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4].
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0015FD=5.23/13055;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0015FD: 1167.67; 1453.9; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WXP=5.15/10500;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WXP: (K)SAGGIVLTGSAAAK(S),21-34;
2D         (K)STRGEVLAVGNGR(I),35-47; (R)GEVLAVGNGR(I),38-47; (K)VGDIVIFNDGYGVK(S),61-74.
2D   -!-   MAPPING: Tandem mass spectrometry [6].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; C015.4; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A6F9; CH10_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli(4.5-5.5)	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)