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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A6P9 |
SWISS-2DPAGE: P0A6P9
P0A6P9:
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ID ENO_ECOLI; STANDARD; 2DG.
AC P0A6P9; P08324;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 6.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate
DE hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase;.
GN Name=eno; OrderedLocusNames=b2779, JW2750;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI6-9, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI6-11, ECOLI6-9, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=83160793; PubMed=6339476; [NCBI, Expasy, EBI, Israel, Japan]
RA Smith M.W., Neidhardt F.C.;
RT ''''''Proteins induced by anaerobiosis in Escherichia coli'';'';'';
RL J. Bacteriol. 154(1):336-343(1983).
RN [6]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [7]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KV0=5.34/46601;
2D -!- PI/MW: SPOT 2D-000KVF=5.29/46325;
2D -!- EXPRESSION: INCREASED DURING ANAEROBIC GROWTH [5].
2D -!- MAPPING: SPOT 2D-000KV0: TAGGING ANALYSIS [1] AND IDENTIFIED ON 2-D
2D GELS BY VANBOGELEN [2] AND BLOCH [3]; SPOT 2D-000KVF: AMINO ACID
2D COMPOSITION AND SEQUENCE TAG (SKIV) [4].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HLY=4.74/22910; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001HLY: 950.682; 1189.748; 1562.908;
2D 1605.966; 1928.974; 2118.107; 2776.085; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014Y3=5.46/45494;
2D -!- PI/MW: SPOT 2D-001523=5.20/40139;
2D -!- PI/MW: SPOT 2D-00157J=5.16/31562;
2D -!- PEPTIDE MASSES: SPOT 2D-0014Y3: 950.662; 1189.819; 1271.862;
2D 1460.115; 1493.139; 1563.142; 1606.202; 1843.27; 1929.296; 2118.528; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001523: 806.44; 1189.6; 1459.88; 1492.93;
2D 1546.82; 1605.95; 1674.95; 1843.01; 2075.22; 2118.18; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-00157J: 1189.605; 1546.81; 1605.904;
2D 1674.884; 1842.908; 2118.003; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6]; SPOT 2D-0014Y3:
2D MICROSEQUENCING (XKIV) [6].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L06=5.29/51580;
2D -!- PI/MW: SPOT 2D-001L0Q=5.27/51301;
2D -!- PI/MW: SPOT 2D-001L0V=5.16/51319;
2D -!- PI/MW: SPOT 2D-001L39=5.00/50698;
2D -!- PI/MW: SPOT 2D-001L3T=5.02/50647;
2D -!- PI/MW: SPOT 2D-001L6G=5.26/50000;
2D -!- PEPTIDE MASSES: SPOT 2D-001L06: 950.606; 1189.757; 1255.782;
2D 1459.977; 1563.017; 1929.113; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L0Q: 888.415; 928.503; 950.43; 1145.492;
2D 1189.527; 1248.519; 1255.537; 1264.527; 1271.535; 1459.725; 1492.729;
2D 1562.731; 1605.769; 1674.784; 1750.681; 1928.782; 2117.906; 2856.118; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L0V: 950.475; 1189.571; 1255.612;
2D 1271.597; 1459.808; 1492.843; 1562.837; 1605.869; 1750.766; 1826.889;
2D 1842.893; 1928.926; 2118.06; 2776.217; 2856.331; 2872.293; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L39: 928.562; 950.51; 1189.619;
2D 1248.607; 1255.651; 1271.616; 1459.83; 1492.886; 1546.832; 1562.848;
2D 1605.893; 1826.912; 1842.875; 1928.93; 2075.116; 2118.046; 2776.22; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L3T: 1255.753; 1605.882; 1826.915;
2D 1928.902; 2775.948; 2856.077; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L6G: 1189.623; 1255.672; 1459.807;
2D 1562.835; 1605.872; 1826.898; 1928.9; 2856.274; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
2D -!- MASTER: ECOLI6-11;
2D -!- PI/MW: SPOT 2D-001TQR=6.95/24005; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001TQR: 1459.912; 1492.946; 1843.015;
2D 2856.6; 2872.631; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
2D -!- MASTER: ECOLI6-9;
2D -!- PI/MW: SPOT 2D-001STX=6.72/25314;
2D -!- MAPPING: GEL MATCHING WITH ECOLI6-11 [6].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WL3=5.35/43401;
2D -!- PEPTIDE MASSES: SPOT 2D-001WL3: 928.49; 950.42; 1189.48; 1255.48;
2D 1459.67; 1492.7; 1562.68; 1605.7; 1675.73; 1928.75; 2117.9; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WL3: (K)AVAAVNGPIAQALIGK(D),66-81;
2D (K)FGANAILAVSLANAK(A),105-119; (K)AFTSEEFTHFLEELTK(Q),266-281;
2D (K)IQLVGDDLFVTNTK(I),311-324; (K)GIANSILIK(F),333-341;
2D (K)FNQIGSLTETLAAIK(M),342-356; (R)IEEALGEK(A),411-418.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [7].
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DR 2DBase-Ecoli; P0A6P9; ENO_ECOLI.
DR ECO2DBASE; F043.8; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A6P9; ENO_ECOLI.
//
The following 2-D maps are available for this protein:
|
Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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|---|
Escherichia coli(5-6)
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Escherichia coli(6-11)
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Escherichia coli(6-9)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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