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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0A7D4 |
SWISS-2DPAGE: P0A7D4
P0A7D4:
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ID PURA_ECOLI; STANDARD; 2DG.
AC P0A7D4; P12283;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=Adenylosuccinate synthetase; Short=AMPSase; Short=AdSS;
DE EC=6.3.4.4; AltName: Full=IMP--aspartate ligase;.
GN Name=purA; Synonyms=adeK; OrderedLocusNames=b4177, JW4135;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WKQ=5.30/44524;
2D -!- PI/MW: SPOT 2D-001WLA=5.30/43494;
2D -!- PEPTIDE MASSES: SPOT 2D-001WKQ: 949.44; 958.54; 1137.55; 1182.72;
2D 2081.18; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WLA: (K)IVDLLTER(A),19-26;
2D (R)YQGGHNAGHTLVINGEK(T),33-49; (K)TVLHLIPSGILR(E),50-61;
2D (R)LLLSEACPLILDYHVALDNAR(E),97-117; (R)GIGPAYEDK(V),132-140;
2D (R)VGDLFDKETFAEK(L),148-160; (K)AEAVDYQK(V),178-185;
2D (R)YVDYVLGILK(A),258-267; (R)TGWLDTVAVR(R),307-316;
2D (R)AVQLNSLSGFCLTK(L),318-331; (K)LDVLDGLKEVK(L),332-342;
2D (R)SGLPQAALNYIK(R),389-400; (K)RIEELTGVPIDIISTGPDR(T),401-419.
2D -!- EXPRESSION: increase after benzoic acid treatment [1].
2D -!- MAPPING: SPOT 2D-001WKQ: Peptide mass fingerprinting [1]; SPOT
2D 2D-001WLA: Tandem mass spectrometry [1].
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CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
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DR UniProtKB/Swiss-Prot; P0A7D4; PURA_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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