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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A910




SWISS-2DPAGE: P0A910



P0A910:

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ID   OMPA_ECOLI; STANDARD; 2DG.
AC   P0A910; P02934;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Outer membrane protein A; AltName: Full=Outer membrane
DE   protein II*; Flags: Precursor;.
GN   Name=ompA; Synonyms=con, tolG, tut; OrderedLocusNames=b0957, JW0940;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: MONOMER (PROBABLE) 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L70=5.55/34492;
2D   -!-   PI/MW: SPOT 2D-000L8I=5.36/32758;
2D   -!-   PI/MW: SPOT 2D-000LEG=5.34/28591;
2D   -!-   MAPPING: GEL MATCHING [1] OR MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LT4=5.23/14346; !FRAGMENT!
2D   -!-   PI/MW: SPOT 2D-001LU0=5.25/13108; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LT4: 872.616; 1083.635; 1157.717;
2D         1214.735; 1222.769; 1280.769; 1370.838; 1378.901; 1409.804; 2600.508; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LU0: 872.527; 1083.557; 1222.675;
2D         1280.675; 1378.789; 1409.693; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WPP=5.50/33875;
2D   -!-   PI/MW: SPOT 2D-001WPU=5.38/33875;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WPP: 915.49; 1055.46; 1083.54; 1155.63;
2D         1214.67; 1280.71; 1795.79; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WPU: (K)AQGVQLTAK(L),95-103;
2D         (K)LGYPITDDLDIYTR(L),104-117; (K)HFTLKSDVLFNFNK(A),214-227;
2D         (K)SDVLFNFNK(A),219-227; (K)ATLKPEGQAALDQLYSQLSNLDPK(D),228-251;
2D         (K)DGSVVVLGYTDRIGSDAYNQGLSER(R),252-276; (R)IGSDAYNQGLSER(R),264-276;
2D         (R)RAQSVVDYLISK(G),277-288; (R)AQSVVDYLISK(G),278-288.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [4].
2D   -!-   MAPPING: SPOT 2D-001WPP: Peptide mass fingerprinting [4]; SPOT
2D         2D-001WPU: Tandem mass spectrometry [4].
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CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
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CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0A910; OMPA_ECOLI.
DR   ECO2DBASE; F033.1; 6TH EDITION.
DR   ECO2DBASE; F024.5; 6TH EDITION.
DR   ECO2DBASE; F028.0; 6TH EDITION.
DR   ECO2DBASE; F033.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A910; OMPA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server