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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P0AA43 |
SWISS-2DPAGE: P0AA43
P0AA43:
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ID RSUA_ECOLI; STANDARD; 2DG.
AC P0AA43; P33918;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 09-NOV-2011, general annotation version 8.
DE RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE EC=5.4.99.19; AltName: Full=16S pseudouridylate 516 synthase; AltName:
DE Full=16S rRNA pseudouridine(516) synthase; AltName: Full=rRNA
DE pseudouridylate synthase A; AltName: Full=rRNA-uridine isomerase A;.
GN Name=rsuA; Synonyms=yejD; OrderedLocusNames=b2183, JW2171;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WR0=5.63/32442;
2D -!- PI/MW: SPOT 2D-001WR8=5.78/32101;
2D -!- PEPTIDE MASSES: SPOT 2D-001WR8: 987.5; 1118.57; 1314.65; 1474.77;
2D 1880.05; 2198.05; 2327.14; 2785.52; 2828.44; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WR0: (K)FIAQQLGVSR(A),6-15; (K)DLTKPAVLEVITPTQVR(L),159-175.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WR8: (K)FIAQQLGVSR(A),6-15;
2D (R)GNRVTVDGEIVR(N),24-35; (K)TYLVTLESPVADDTAEQFAK(G),131-150; (K)DLTKPAVLEVITPTQVR(L),159-175.
2D -!- MAPPING: Tandem mass spectrometry [1]; SPOT 2D-001WR8: PEPTIDE MASS
2D FINGERPRINTING [1].
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DR UniProtKB/Swiss-Prot; P0AA43; RSUA_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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