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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P11142 |
SWISS-2DPAGE: P11142
P11142:
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ID HSP7C_HUMAN; STANDARD; 2DG.
AC P11142;
DT 15-JAN-1999, integrated into SWISS-2DPAGE (release 9).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Heat shock cognate 71 kDa protein; AltName: Full=Heat shock
DE 70 kDa protein 8;.
GN Name=HSPA8; Synonyms=HSC70, HSP73, HSPA10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Hominidae; Homo.
OX NCBI_TaxID=9606;
MT DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM DLD1_HUMAN, NUCLEI_LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN [1]
RP MAPPING ON GEL.
RA Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA Appel R.D., Hochstrasser D.F.;
RL Submitted (JAN-1999) to SWISS-2DPAGE.
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan]
RA Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL Electrophoresis 21(1):3483-3487(2000).
RN [3]
RP MAPPING ON GEL.
RX PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL Mol. Biol. Cell. 13(1):4100-4109(2002).
2D -!- MASTER: DLD1_HUMAN;
2D -!- PI/MW: SPOT 2D-001DK7=5.17/69894;
2D -!- PEPTIDE MASSES: SPOT 2D-001DK7: 1253.53; 1481.7; 1487.59; 1981.83; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: NUCLEI_LIVER_HUMAN;
2D -!- PI/MW: SPOT 2D-001J00=5.39/67478;
2D -!- PI/MW: SPOT 2D-001J03=5.31/67478;
2D -!- PEPTIDE MASSES: SPOT 2D-001J00: 861.415; 1074.472; 1081.553;
2D 1199.682; 1228.633; 1235.634; 1251.64; 1253.63; 1426.68; 1481.81;
2D 1487.708; 1659.848; 1691.739; 1773.9; 1982.006; 2774.294; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001J03: 861.437; 882.408; 993.495;
2D 1074.491; 1081.569; 1175.629; 1199.693; 1228.643; 1235.622; 1251.612;
2D 1253.635; 1303.613; 1319.643; 1410.693; 1426.687; 1481.828; 1487.733;
2D 1616.792; 1632.807; 1649.822; 1653.857; 1659.886; 1665.848; 1691.752;
2D 1982.007; 2260.15; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [2].
2D -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D -!- PI/MW: SPOT 2D-001VGX=5.50/70432;
2D -!- PEPTIDE MASSES: SPOT 2D-001VGX: 1081.7; 1197.8; 1199.8; 1228.8;
2D 1251.8; 1253.8; 1426.8; 1480.9; 1487.9; 1632.9; 1666; 1691.9; 1746;
2D 1788.1; 1982.2; 2263.4; 2697.6; 2774.6; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001VGX:
2D (K)GPAVGIDLGTTYSCVGVFQHGK(V),4-25; (K)VEIIANDQGNR(T),26-36;
2D (R)TTPSYVAFTDTER(L),37-49; (R)RFDDAVVQSDMoxK(H),77-88;
2D (K)SFYPEEVSSMoxVLTK(M),113-126; (K)TVTNAVVTVPAYFNDSQR(Q),138-155;
2D (K)DAGTIAGLNVLR(I),160-171; (R)IINEPTAAAIAYGLDKK(V),172-188;
2D (R)GTLDPVEK(A),312-319; (K)NSLESYAFNMoxK(A),540-550.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [3].
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DR DOSAC-COBS 2D-PAGE; P11142; P11142.
DR DOSAC-COBS-2DPAGE; P11142; HSP7C_HUMAN.
DR HSC-2DPAGE; P11142; HUMAN.
DR OGP-WWW; P11142.
DR PHCI-2DPAGE; P11142; HS7C_HUMAN.
DR REPRODUCTION-2DPAGE; IPI00003865; IPI00003865.
DR Siena-2DPAGE; P11142; HSP7C_HUMAN.
DR UCD-2DPAGE; P11142; HSP7C_HUMAN.
DR UniProtKB/Swiss-Prot; P11142; HSP7C_HUMAN.
//
The following 2-D maps are available for this protein:
|
Colorectal adenocarcinoma cell line (DL-1)
 |
Soluble nuclear proteins and matrix from liver tissue
 |
2D-PAGE of nucleolar proteins from Human HeLa cells
 |
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SWISS-2DPAGE (search AC)
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