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Sample Preparation and Post-separation Analysis

P17742:

ID   PPIA_MOUSE; STANDARD; 2DG.
AC   P17742;
DT   01-SEP-1998, integrated into SWISS-2DPAGE (release 7).
DT   01-OCT-2001, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 9.
DE   RecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A;
DE   EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin
DE   A-binding protein; AltName: Full=Rotamase A; AltName: Full=SP18;.
GN   Name=Ppia;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
MT   BAT_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
IM   BAT_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano
RA   S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A.,
RA   Hochstrasser D.F.;
RT   ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of
RT   diabetes and obesity'';'';'';
RL   Proteomics 1(1):136-163(2001).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11503206; [NCBI, Expasy, EBI, Israel, Japan]
RA   Lanne B., Potthast F., Hoglund A., Brockenhuus Von Lowenhielm H., Nystrom
RA   A.-C., Nilsson F., Dahllof B.;
RT   ''''''Thiourea enhances mapping of the proteome from murine white adipose tissue'';'';'';
RL   Proteomics 1(1):819-828(2001).
RN   [3]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Sanchez J.-C.;
RL   Submitted (OCT-2001) to SWISS-2DPAGE.
2D   -!- MASTER: BAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001QL6=7.75/14235;
2D   -!-   MAPPING: GEL MATCHING WITH WAT_MOUSE [1].
2D   -!- MASTER: LIVER_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0014IC=7.18/15283;
2D   -!-   PI/MW: SPOT 2D-0014IH=7.60/15061;
2D   -!-   PI/MW: SPOT 2D-0014IM=7.01/14967;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014IC: 1055.464; 1154.504; 1310.527;
2D         1379.728; 1614.688; 1831.821; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014IH: 1055.53; 1154.597; 1614.73;
2D         2005.949; TRYPSIN.
2D   -!-   MAPPING: SPOTS 2D-0014IC, 2D-0014IH: Peptide mass fingerprinting
2D         [1]; SPOTS 2D-0014IH,2D-0014IM: MICROSEQUENCING [1].
2D   -!- MASTER: MUSCLE_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0017RA=6.93/14967;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0017RA: 1006.471; 1055.54; 1154.576;
2D         1310.61; 1614.749; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: WAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001CDY=7.04/13816;
2D   -!-   PI/MW: SPOT 2D-001CEH=7.46/13456;
2D   -!-   PI/MW: SPOT 2D-001CEM=6.97/13338;
2D   -!-   MAPPING: SPOT 2D-001CDY: GEL MATCHING [3] WITH WAT_MOUSE 2-DE MAP
2D         FROM LANNE  ET AL [2]; SPOTS 1CE*: MATCHING WITH THE MOUSE LIVER MASTER
2D         GEL [1].
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DR   REPRODUCTION-2DPAGE; P17742; P17742.
DR   UCD-2DPAGE; P17742; PPIA_MOUSE.
DR   UniProtKB/Swiss-Prot; P17742; PPIA_MOUSE.
//

The following 2-D maps are available for this protein:

	Brown adipose tissue	Liver	Gastrocnemius muscle
	White adipose tissue

SWISS-2DPAGE (search AC)