Sample Preparation and Post-separation Analysis

P24534:

ID   EF1B_HUMAN; STANDARD; 2DG.
AC   P24534;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Elongation factor 1-beta; Short=EF-1-beta;.
GN   Name=EEF1B2; Synonyms=EEF1B, EF1B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN,
MT   LYMPHOMA_HUMAN, U937_HUMAN.
IM   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN,
IM   LYMPHOMA_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [5]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
CC   -!- SUBUNIT: ALPHA, BETA, DELTA, AND GAMMA 
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000UCG=4.54/30421;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [4].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001ENE=4.50/28086;
2D   -!-   MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [5].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000EN3=4.55/28063;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0003I4=4.51/28602;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000NK7=4.57/28635;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000197=4.53/28206;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00081C=4.54/28196;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D2X=4.47/29497;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
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DR   DOSAC-COBS 2D-PAGE; P24534; P24534.
DR   DOSAC-COBS-2DPAGE; P24534; EF1B_HUMAN.
DR   OGP-WWW; P24534.
DR   PHCI-2DPAGE; P24534; EF1B_HUMAN.
DR   Siena-2DPAGE; P24534; EF1B_HUMAN.
DR   UniProtKB/Swiss-Prot; P24534; EF1B_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal epithelia cells	Colorectal adenocarcinoma cell line (DL-1)	Erythroleukemia Cell
	HepG2	Kidney	Liver
	Lymphoma	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)