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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P28635 |
SWISS-2DPAGE: P28635
P28635:
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ID METQ_ECOLI; STANDARD; 2DG.
AC P28635;
DT 01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 9.
DE RecName: Full=D-methionine-binding lipoprotein metQ; Flags: Precursor;.
GN Name=metQ; Synonyms=yaeC; OrderedLocusNames=b0197, JW0193;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4-5, ECOLI-DIGE4.5-6.5.
IM ECOLI4-5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HOB=4.95/11934; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001HOI=4.95/11539; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001HOB: 1264.738; 1290.716; 1861.846;
2D 1989.911; 2194.134; 2325.126; 2674.368; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HOI: 1264.724; 1290.703; 1861.885;
2D 1989.982; 2194.196; 2325.203; 2674.464; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WSM=4.90/27000;
2D -!- PEPTIDE MASSES: SPOT 2D-001WSM: 1264.61; 1290.58; 1724.79; 1798.79;
2D 1861.68; 2005.93; 2193.94; 2324.93; 2471.98; 2566.06; 2634.02; 2674.15;
2D 2742.11; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WSM:
2D (K)DKYGLDVELVTENDYVLPNFALSK(G),55-78; (K)LVAVGNTFVYPIAGYSK(K),103-119; (K)LKDGVGLLPTVLDVVENPK(N),157-175.
2D -!- EXPRESSION: decrease after benzoic acid treatment [2].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [2].
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DR 2DBase-Ecoli; P28635; METQ_ECOLI.
DR UniProtKB/Swiss-Prot; P28635; METQ_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4-5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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