Sample Preparation and Post-separation Analysis

P30041:

ID   PRDX6_HUMAN; STANDARD; 2DG.
AC   P30041; P32077;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 3.
DT   09-NOV-2011, general annotation version 14.
DE   RecName: Full=Peroxiredoxin-6; EC=1.11.1.15; AltName: Full=1-Cys
DE   peroxiredoxin; Short=1-Cys PRX; AltName: Full=24 kDa protein; AltName:
DE   Full=Acidic calcium-independent phospholipase A2; Short=aiPLA2;
DE   EC=3.1.1.-; AltName: Full=Antioxidant protein 2; AltName: Full=Liver 2D
DE   page spot 40; AltName: Full=Non-selenium glutathione peroxidase;
DE   Short=NSGPx; EC=1.11.1.9; AltName: Full=Red blood cells page spot 12;.
GN   Name=PRDX6; Synonyms=AOP2, KIAA0106;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, HEPG2_HUMAN, HL60_HUMAN, LIVER_HUMAN, LYMPHOCYTE_HUMAN,
MT   LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
IM   DLD1_HUMAN, HEPG2_HUMAN, HL60_HUMAN, LIVER_HUMAN, LYMPHOCYTE_HUMAN,
IM   LYMPHOMA_HUMAN, RBC_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, Expasy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [5]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [6]
RP   MAPPING ON GEL.
RX   MEDLINE=94147970; PubMed=8313871; [NCBI, Expasy, EBI, Israel, Japan]
RA   Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C.,
RA   Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P.,
RA   Hochstrasser D.F.;
RT   ''''''Plasma and red blood cell protein maps: update 1993'';'';'';
RL   Electrophoresis 14(1):1223-1231(1993).
RN   [7]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001ER2=6.17/25202;
2D   -!-   MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [5].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0003M9=6.28/25861;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000ZM3=6.35/23368;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3][4].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001C0=6.27/25710;
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YD5=6.73/25482;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001YD5: 906.4809; 1045.564; 1085.6079;
2D         1191.692; 1395.6768; 2098.0915; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [7].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00083K=6.26/25930;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: RBC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0006I4=6.45/25743;
2D   -!-   MAPPING: MICROSEQUENCING [6].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D45=6.27/25748;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
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DR   DOSAC-COBS 2D-PAGE; P30041; P30041.
DR   DOSAC-COBS-2DPAGE; P30041; PRDX6_HUMAN.
DR   OGP-WWW; P30041.
DR   REPRODUCTION-2DPAGE; IPI00220301; IPI00220301.
DR   REPRODUCTION-2DPAGE; P30041; P30041.
DR   Siena-2DPAGE; P30041; PRDX6_HUMAN.
DR   UniProtKB/Swiss-Prot; P30041; PRDX6_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)	HepG2	Promyelocytic leukemia cells
	Liver	Lymphocytes	Lymphoma
	Red blood cells	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)