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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P30126 |
SWISS-2DPAGE: P30126
P30126:
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ID LEUD_ECOLI; STANDARD; 2DG.
AC P30126;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 6.
DE RecName: Full=3-isopropylmalate dehydratase small subunit; EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName:
DE Full=Isopropylmalate isomerase;.
GN Name=leuD; OrderedLocusNames=b0071, JW0070;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: CONSISTS OF TWO DIFFERENT SUBUNITS: LEUC AND LEUD
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WV1=5.20/23000;
2D -!- PEPTIDE MASSES: SPOT 2D-001WV1: 869.41; 1025.44; 1420.49; 1543.49;
2D 1548.56; 1881.63; 2158.79; 2672.86; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WV1:
2D (K)GQQPNPDFVLNFPQYQGASILLAR(E),54-77;
2D (K)VVIAPSFADIFYGNSFNNQLLPVK(L),100-123; (K)LSDAEVDELFALVK(A),124-137.
2D -!- EXPRESSION: decrease after benzoic acid treatment [1].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P30126; LEUD_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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