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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P37689




SWISS-2DPAGE: P37689



P37689:

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ID   GPMI_ECOLI; STANDARD; 2DG.
AC   P37689;
DT   01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=2,3-bisphosphoglycerate-independent phosphoglycerate
DE   mutase; Short=BPG-independent PGAM; Short=Phosphoglyceromutase;
DE   Short=iPGM; EC=5.4.2.1;.
GN   Name=gpmI; Synonyms=pgmI, yibO; OrderedLocusNames=b3612, JW3587;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014VM=5.15/52457;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014VM: 1007.693; 1204.76; 1329.72;
2D         1449.819; 1639.908; 2312.096; 2329.931; 2827.165; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WI2=5.15/52681;
2D   -!-   PI/MW: SPOT 2D-001WIC=5.11/52363;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WI2: 861.35; 874.42; 987.46; 995.32;
2D         1007.46; 1204.51; 1329.47; 1449.56; 1569.57; 1625.63; 1770.7; 1978.64;
2D         2435.82; 2514.98; 2827.06; 2984.07; 3026.98; 3043.99; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WI2: (R)AFFANPVLTGAVDK(A),92-105;
2D         (K)AYDLLTLAQGEFQADTAVAGLQAAYAR,203-229; (R)DENDEFVK(A),230-237;
2D         (R)AFVNADFDGFAR(K),273-284; (K)YAHVTFFFNGGVEESFKGEDR(I),337-357.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WIC: (R)EEQQDNAIFSAK(T),20-31;
2D         (R)AFFANPVLTGAVDK(A),92-105; (K)AYDLLTLAQGEFQADTAVAGLQAAYAR,203-229; (R)AFVNADFDGFAR(K),273-284.
2D   -!-   MAPPING: SPOT 2D-001WI2: PEPTIDE MASS FINGERPRINTING [2]; Tandem
2D         mass spectrometry [2].
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CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
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DR   UniProtKB/Swiss-Prot; P37689; GPMI_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server