World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P63284




SWISS-2DPAGE: P63284



P63284:

Nice View - a user-friendly view of this entry
ID   CLPB_ECOLI; STANDARD; 2DG.
AC   P63284; P03815;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Chaperone protein ClpB; AltName: Full=Heat shock protein F84.1;.
GN   Name=clpB; Synonyms=htpM; OrderedLocusNames=b2592, JW2573;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Gage D.J., Neidhardt F.C.;
RT   ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT   phosphorylation 2,4-dinitrophenol'';'';'';
RL   J. Bacteriol. 175(1):7105-7108(1993).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: THOUGHT TO BE AN ATPASE SUBUNIT OF AN INTRACELLULAR
CC         ATP-DEPENDENT PROTEASE
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KEW=5.30/88421;
2D   -!-   PI/MW: SPOT 2D-000KI3=5.38/73900;
2D   -!-   EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LEJ=5.44/34853; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LEJ: 915.399; 955.354; 971.372;
2D         1079.384; 1095.377; 1145.348; 1187.473; 1210.441; 1226.429; 1286.568;
2D         1560.698; 1655.694; 1732.586; 1748.565; 1921.823; 1989.81; 2005.79;
2D         2125.913; 2283.991; 2296.898; 2684.143; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WC2=5.33/88745;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WC2: 857.5; 956.48; 1010.47; 1057.55;
2D         1070.57; 1085.45; 1187.57; 1335.65; 1373.69; 1381.73; 1384.71; 1528.82;
2D         1560.84; 1748.76; 1758.93; 1774.93; 1955.03; 2006.04; 2048.13; 2124.11;
2D         2284.25; 2297.15; 2453.23; 2474.36; 3020.55; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WC2: (R)IINGEVPEGLKGR(R),223-235; (K)VFVAEPSVEDTIAILR(G),336-351.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [5].
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [5].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; E072.0; 6TH EDITION.
DR   ECO2DBASE; F084.1; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P63284; CLPB_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server