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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P99063 |
SWISS-2DPAGE: P99063
P99063:
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ID ODPB_STAAN; STANDARD; 2DG.
AC P99063;
DT 31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 4.
DE RecName: Full=Pyruvate dehydrogenase E1 component subunit beta; EC=1.2.4.1;.
GN Name=pdhB; OrderedLocusNames=SA0944;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX NCBI_TaxID=158879;
MT STAPHYLOCOCCUS.
IM STAPHYLOCOCCUS.
RN [1]
RP MAPPING ON GEL.
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT ''''''Correlation of proteomic and transcriptomic profiles of
RT Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL J. Microbiol. Methods 60(2):247-257(2005).
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain
2D -!- MASTER: STAPHYLOCOCCUS;
2D -!- PI/MW: SPOT 2D-001XZP=5.01/33544;
2D -!- PI/MW: SPOT 2D-001XZS=4.95/33439;
2D -!- PI/MW: SPOT 2D-001XZT=4.90/33596;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XZP: (M)AQMTMVQAINDALK(T),2-15;
2D (K)VVIPSGPYDAK(G),145-155; (R)SNDPVVYLEHMK(L),164-175;
2D (R)SNDPVVYLEHMoxK(L),164-175; (R)EEVPEEEYTIDIGK(A),182-195;
2D (K)AAEELEKDGYSVEVIDLR(T),220-237; (R)TVQPIDVDTIVASVEK(T),238-253;
2D (R)AVVVQEAQR(Q),257-265; (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XZS: (R)SFREEVPEEEYTIDIGK(A),179-195;
2D (R)EEVPEEEYTIDIGK(A),182-195; (R)TVQPIDVDTIVASVEK(T),238-253;
2D (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XZT: (K)VVIPSGPYDAK(G),145-155;
2D (R)SFREEVPEEEYTIDIGK(A),179-195; (R)AVVVQEAQR(Q),257-265;
2D (R)QAGVGAAVVAELSER(A),266-280; (R)AILSLEAPIGR(V),281-291.
2D -!- MAPPING: MALDI tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P99063; ODPB_STAAN.
//
The following 2-D map is available for this protein:
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Staphylococcus aureus
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SWISS-2DPAGE (search AC)
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