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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P99083 |
SWISS-2DPAGE: P99083
P99083:
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ID CH60_STAAN; STANDARD; 2DG.
AC P99083;
DT 31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 5.
DE RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName:
DE Full=Protein Cpn60;.
GN Name=groL; Synonyms=groEL; OrderedLocusNames=SA1836;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX NCBI_TaxID=158879;
MT STAPHYLOCOCCUS.
IM STAPHYLOCOCCUS.
RN [1]
RP MAPPING ON GEL.
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT ''''''Correlation of proteomic and transcriptomic profiles of
RT Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL J. Microbiol. Methods 60(2):247-257(2005).
CC -!- SUBUNIT: Oligomer of 14 subunits composed of two stacked rings of 7
CC subunits (By similarity)
2D -!- MASTER: STAPHYLOCOCCUS;
2D -!- PI/MW: SPOT 2D-001XSX=4.78/67907;
2D -!- PI/MW: SPOT 2D-001XW1=4.31/42839;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XSX: (R)GVDQLANAVK(V),18-27;
2D (K)EIELEDPYENMGAK(L),58-71; (K)NVTSGANPVGLR(Q),105-116;
2D (K)VENKNEIAQVGAISAADEEIGR(Y),137-158; (K)NEIAQVGAISAADEEIGR(Y),141-158;
2D (R)GYQSPYMVTDSDK(M),196-208; (K)MVAELERPYILVTDKK(I),209-224;
2D (R)AAVEEGIVAGGGTALVNVYQK(V),403-423; (R)QIAENAGLEGSVIVER(L),450-465.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XW1: (K)SQIEETESDFDREK(L),349-362.
2D -!- PEPTIDE MASSES: 1018.51 [1]; 1289.6625 [1]; 1323.6603 [1]; 1340.666
2D [1]; 1490.6554 [1]; 1493.7301 [1]; 1497.7504 [1]; 1506.6384 [1];
2D 1507.7013 [1]; 1684.8727 [1]; 1712.75 [1]; 1813.6825 [1]; 1892.7921 [1];
2D 2046.0719 [1]; 2449.1025 [1]; TRYPSIN [1].
2D -!- MAPPING: MALDI tandem mass spectrometry [1]; SPOT 2D-001XW1:
2D Peptide mass fingerprinting [1].
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DR UniProtKB/Swiss-Prot; P99083; CH60_STAAN.
//
The following 2-D map is available for this protein:
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Staphylococcus aureus
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SWISS-2DPAGE (search AC)
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