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Sample Preparation and Post-separation Analysis

P99100:

ID   DEOB_STAAN; STANDARD; 2DG.
AC   P99100;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 5.
DE   RecName: Full=Phosphopentomutase; EC=5.4.2.7; AltName: Full=Phosphodeoxyribomutase;.
GN   Name=deoB; Synonyms=drm; OrderedLocusNames=SA0134;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XVT=4.86/43532;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XVT:
2D         (K)LGLGNIDKLPVVNAVEQPEAYYTK(L),52-75; (R)IIARPYVGEPGNFTR(T),194-208;
2D         (R)TSNRHDYALKPFGK(T),209-222; (K)INDIYDGEGVTEAVR(T),241-255;
2D         (K)DFTGISFLNLVDFDALYGHR(R),273-292; (R)DKPGYAQAIK(D),294-303; (R)EYIPVIMYSPK(F),342-352.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99100; DEOB_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)