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Sample Preparation and Post-separation Analysis

P99112:

ID   ATPB_STAAN; STANDARD; 2DG.
AC   P99112;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 4.
DE   RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP
DE   synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta;.
GN   Name=atpD; OrderedLocusNames=SA1905;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
CC   -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC         - and CF(0) - the membrane proton channel. CF(1) has five subunits:
CC         alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main
CC         subunits: a, b and c
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XVM=4.99/43756;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XVM: (R)VTQVMGPVIDVR(F),6-17;
2D         (K)EISVPVGDETLGR(V),79-91; (K)VVDLLAPYIK(G),138-147;
2D         (R)FTQAGSEVSALLGR(M),254-267; (R)ALEPSIVGQEHYEVAR(D),350-365;
2D         (R)ALEPSIVGQEHYEVAR(D),350-365; (K)DILDGKYDHIPEDAFR(L),436-451;
2D         (K)YDHIPEDAFR(L),442-451; (K)YDHIPEDAFR(L),442-451.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99112; ATPB_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)