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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P99134 |
SWISS-2DPAGE: P99134
P99134:
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ID SPA_STAAN; STANDARD; 2DG.
AC P99134;
DT 31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 5.
DE RecName: Full=Immunoglobulin G-binding protein A; Short=IgG-binding
DE protein A; AltName: Full=Staphylococcal protein A; Flags: Precursor;.
GN Name=spa; OrderedLocusNames=SA0107;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX NCBI_TaxID=158879;
MT STAPHYLOCOCCUS.
IM STAPHYLOCOCCUS.
RN [1]
RP MAPPING ON GEL.
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT ''''''Correlation of proteomic and transcriptomic profiles of
RT Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL J. Microbiol. Methods 60(2):247-257(2005).
2D -!- MASTER: STAPHYLOCOCCUS;
2D -!- PI/MW: SPOT 2D-001XUZ=5.12/52649;
2D -!- PI/MW: SPOT 2D-001XVB=4.90/49246;
2D -!- PI/MW: SPOT 2D-001XVR=4.91/43532;
2D -!- PI/MW: SPOT 2D-001XW6=5.11/42441;
2D -!- PI/MW: SPOT 2D-001XWJ=5.03/41719;
2D -!- PI/MW: SPOT 2D-001XWL=4.92/41525;
2D -!- PEPTIDE MASSES: SPOT 2D-001XVB: 1014.4423; 1029.9977; 1065.9648;
2D 1149.3989; 1254.9945; 1614.7119; 1634.6479; 1913.8451; 2064.8398;
2D 2347.9429; 2348.9395; 2361.9319; 2397.8855; 2445.984; 2445.9844;
2D 2446.9802; 2484.9519; 2495.9075; 3299.3213; 3512.3105; 3527.3569; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001XVR: 1014.4831; 1030.0453; 1065.4497;
2D 1066.0043; 1149.4529; 1193.5341; 1586.6492; 1598.7887; 1614.7849;
2D 1634.7253; 1867.7946; 1913.9094; 2064.9087; 2348.0239; 2363.0061;
2D 2396.9595; 2446.076; 2446.0764; 2447.0593; 2485.042; 2495.9917; 2890.24;
2D 3300.302; 3512.4221; 3527.4089; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001XW6: 1014.469; 1045.4745; 1059.9633;
2D 1065.4244; 1149.4379; 1193.5132; 1194.5048; 1316.4243; 1332.4125;
2D 1391.5668; 1475.6592; 1475.9655; 1558.604; 1614.7527; 1634.7025;
2D 1635.6969; 1689.7614; 1913.8889; 2009.9066; 2063.8706; 2064.8757;
2D 2347.9871; 2362.0024; 2383.7791; 2396.926; 2398.9099; 2446.0266;
2D 2446.027; 2484.991; 2495.9626; 2890.2192; 3527.3608; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001XWJ: 1014.4932; 1044.0219; 1065.4508;
2D 1066.0089; 1149.4664; 1193.5435; 1254.5291; 1460.709; 1558.6534;
2D 1598.8081; 1614.7946; 1634.7474; 1726.7419; 1913.9299; 2063.9387;
2D 2064.9402; 2348.0527; 2348.053; 2349.0481; 2362.0513; 2396.9856;
2D 2446.093; 2447.0867; 2485.0781; 2496.0117; 2889.2561; 3300.3604;
2D 3527.4844; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001XWL: 1014.5282; 1065.4854; 1149.5038;
2D 1193.5741; 1460.6937; 1586.7307; 1598.8419; 1614.8414; 1634.802;
2D 1913.9836; 2064.0002; 2348.1201; 2363.1099; 2397.0574; 2446.1628;
2D 2446.163; 2447.1621; 2485.1245; 2496.0864; 2890.3633; 3528.5125; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XUZ:
2D (R)NGFIQSLKDDPSQSANVLGEAQK(L),62-84;
2D (R)NGFIQSLKDDPSQSTNVLGEAK(K),123-144; (K)EQQNAFYEILHLPNLNEEQR(N),219-238.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XVB: (R)NGFIQSLKDDPSQSANVLGEAQK(L),62-84.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XVR: (R)NGFIQSLKDDPSQSANVLGEAQK(L),62-84.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XW6: (R)NGFIQSLKDDPSQSANVLGEAQK(L),62-84.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XWJ: (R)NGFIQSLKDDPSQSTNVLGEAK(K),123-144.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XWL: (R)NGFIQSLKDDPSQSANVLGEAQK(L),62-84.
2D -!- MAPPING: MALDI tandem mass spectrometry, and peptide mass
2D fingerprinting [1].
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DR UniProtKB/Swiss-Prot; P99134; SPA_STAAN.
//
The following 2-D map is available for this protein:
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Staphylococcus aureus
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SWISS-2DPAGE (search AC)
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