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Sample Preparation and Post-separation Analysis

P99169:

ID   GATB_STAAN; STANDARD; 2DG.
AC   P99169;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 4.
DE   RecName: Full=Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B;
DE   Short=Asp/Glu-ADT subunit B; EC=6.3.5.-;.
GN   Name=gatB; OrderedLocusNames=SA1715;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
CC   -!- SUBUNIT: Heterotrimer of A, B and C subunits (By similarity) 
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XV2=4.97/51313;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XV2: (K)NYFYPDNPK(A),80-88;
2D         (K)STHKGEYSLVDLNR(Q),130-143; (R)QGTPLIEIVSEPDIR(S),144-158;
2D         (K)EAYAYLEK(L),162-169; (R)CDANISLRPYGQEK(F),191-204;
2D         (K)NLNSFNYVR(K),213-221; (K)RQEEELLNGGEIGQETR(R),230-246;
2D         (R)QTIPELPDER(K),290-299; (K)GQANPQLVNQLLK(Q),457-469.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99169; GATB_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)