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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: POTD_ECOLI




SWISS-2DPAGE: POTD_ECOLI



POTD_ECOLI:

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ID   POTD_ECOLI; STANDARD; 2DG.
AC   P0AFK9; P23861;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Spermidine/putrescine-binding periplasmic protein;
DE   Short=SPBP; Flags: Precursor;.
GN   Name=potD; OrderedLocusNames=b1123, JW1109;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L5Q=4.76/35885;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HK9=4.95/35000;
2D   -!-   PI/MW: SPOT 2D-001HKA=4.76/34885;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HK9: 993.558; 1009.53; 1060.587;
2D         1085.568; 1150.611; 1179.62; 1307.712; 1310.725; 1398.894; 1700.922;
2D         1709.878; 1798.834; 1804.888; 1814.835; 1819.917; 2197.09; 2451.05; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HKA: 993.512; 1009.51; 1060.564;
2D         1179.603; 1398.835; 1700.874; 1804.87; 1814.784; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3]; SPOT 2D-001HK9: GEL
2D         MATCHING WITH MASTER ECOLI4.5-5.5 [3].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-00155G=4.96/35058;
2D   -!-   PEPTIDE MASSES: SPOT 2D-00155G: 993.47; 1009.47; 1060.54; 1150.56;
2D         1179.56; 1307.64; 1398.82; 1700.86; 1804.84; 1814.78; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3], and microsequencing
2D         (XDNN) [3].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WP0=4.88/35000;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WP0: (K)GSLLLTDDAR(E),161-170;
2D         (R)QAGTPIDVVWPK(E),238-249; (K)LINFLLRPDVAK(Q),274-285.
2D   -!-   MAPPING: Tandem mass spectrometry [4].
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DR   2DBase-Ecoli; P0AFK9; POTD_ECOLI.
DR   UniProtKB/Swiss-Prot; P0AFK9; POTD_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server