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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: PTFAH_ECOLI |
SWISS-2DPAGE: PTFAH_ECOLI
PTFAH_ECOLI:
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ID PTFAH_ECOLI; STANDARD; 2DG.
AC P69811; P24217;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=Multiphosphoryl transfer protein; Short=MTP; AltName:
DE Full=Phosphotransferase FPr protein; AltName: Full=Pseudo-HPr; Includes:
DE RecName: Full=Phosphocarrier protein HPr; Short=Protein H; Includes:
DE RecName: Full=Fructose-specific phosphotransferase enzyme IIA component;
DE EC=2.7.1.-; AltName: Full=PTS system fructose-specific EIIA component;.
GN Name=fruB; Synonyms=fpr, fruF; OrderedLocusNames=b2169, JW2156;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WN5=4.85/38938;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WN5: (K)SATTAEELR(A),128-136;
2D (R)LKEAGAVDATFVTK(A),175-188; (R)LADLLLDNKADR(A),251-262.
2D -!- EXPRESSION: decrease after benzoic acid treatment [1].
2D -!- MAPPING: Tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P69811; PTFAH_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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