Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

Sample Preparation and Post-separation Analysis

Q15233:

ID   NONO_HUMAN; STANDARD; 2DG.
AC   Q15233;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   15-MAY-2003, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=Non-POU domain-containing octamer-binding protein;
DE   Short=NonO protein; AltName: Full=54 kDa nuclear RNA-and DNA-binding
DE   protein; AltName: Full=55 kDa nuclear protein; AltName: Full=DNA-binding
DE   p52/p100 complex, 52 kDa subunit; AltName: Full=NMT55; AltName:
DE   Full=p54(nrb); Short=p54nrb;.
GN   Name=NONO; Synonyms=NRB54;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8F=59243;
1D   -!-   PEPTIDE MASSES: BAND 1D-001V8F: 939.5; 1016.5; 1086.6; 1102.6;
1D         1147.6; 1180.6; 1196.6; 1208.6; 1228.6; 1231.7; 1540.8; 1695.7; 1711.7;
1D         1859.9; 1875.9; 2163.1; TRYPSIN.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8F: (R)LFVGNLPPDITEEEMR(K),75-90;
1D         (R)FAQPGSFEYEYAMoxR(W),256-269; (R)FAQPGSFEYEYAMR(W),256-269; (R)EQEIR(M),378-382.
1D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass SPECTROMETRY [1].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   REPRODUCTION-2DPAGE; IPI00304596; IPI00304596.
DR   UniProtKB/Swiss-Prot; Q15233; NONO_HUMAN.
//

The following 2-D map is available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)