Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

Q9GZL7:

ID   WDR12_HUMAN; STANDARD; 2DG.
AC   Q9GZL7;
DT   15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT   15-MAY-2003, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 6.
DE   RecName: Full=Ribosome biogenesis protein WDR12; AltName: Full=WD
DE   repeat-containing protein 12;.
GN   Name=WDR12;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8K=52788;
1D   -!-   MW: BAND 1D-001V8L=51716;
1D   -!-   MW: BAND 1D-001V8M=50927;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8K: (K)SIMoxTIVGHTDVVK(D),132-144; (K)SIMTIVGHTDVVK(D),132-144.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8L:
1D         (K)YAVDDVPFSIPAASEIADLSNIINK(L),15-39; (K)HVEFDFLIK(G),50-58;
1D         (K)SIMTIVGHTDVVK(D),132-144; (K)VFNCISYSPLCK(R),299-310;
1D         (K)WSPTHEQQLISGSLDNIVK(L),350-368; (K)VLSVDWTDTGLLLSGGADNK(L),389-408.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8M: (K)HVEFDFLIK(G),50-58;
1D         (K)SIMoxTIVGHTDVVK(D),132-144; (K)SIMTIVGHTDVVK(D),132-144;
1D         (R)VWDVESGSLK(S),282-291; (K)VFNCISYSPLCK(R),299-310;
1D         (K)APLYDLAAHEDK(V),377-388; (K)VLSVDWTDTGLLLSGGADNK(L),389-408.
1D   -!-   MAPPING: Tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; Q9GZL7; WDR12_HUMAN.
//

The following 2-D map is available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)