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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: SYFA_ECOLI |
SWISS-2DPAGE: SYFA_ECOLI
SYFA_ECOLI:
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ID SYFA_ECOLI; STANDARD; 2DG.
AC P08312;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 01-OCT-2001, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Phenylalanyl-tRNA synthetase alpha chain; EC=6.1.1.20;
DE AltName: Full=Phenylalanine--tRNA ligase alpha chain; Short=PheRS;.
GN Name=pheS; OrderedLocusNames=b1714, JW5277;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5.5-6.7, ECOLI5-6.
IM ECOLI, ECOLI5.5-6.7, ECOLI5-6.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=79216190; PubMed=156716; [NCBI, Expasy, EBI, Israel, Japan]
RA Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.;
RT ''''''Levels of major proteins of Escherichia coli during growth at
RT different temperatures'';'';'';
RL J. Bacteriol. 139(1):185-194(1979).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=78212912; PubMed=352533; [NCBI, Expasy, EBI, Israel, Japan]
RA Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.;
RT ''''''Patterns of protein synthesis in E. coli: a catalog of the amount
RT of 140 individual proteins at different growth rates'';'';'';
RL Cell 14(1):179-190(1978).
RN [6]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
CC -!- SUBUNIT: TETRAMER OF TWO ALPHA AND TWO BETA CHAINS
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000L2X=5.85/38765;
2D -!- EXPRESSION: CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS
2D TEMPERATURE RANGE (23 TO 37°C) [4]; INCREASED IN LEVEL WITH INCREASING
2D GROWTH RATE [5].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D [2] AND BLOCH [3].
2D -!- MASTER: ECOLI5.5-6.7;
2D -!- PI/MW: SPOT 2D-001UBR=5.88/40109;
2D -!- MAPPING: GEL MATCHING WITH ECOLI5-6 [6].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L8E=5.81/45820;
2D -!- PI/MW: SPOT 2D-001LAE=5.74/40430;
2D -!- PEPTIDE MASSES: SPOT 2D-001L8E: 823.451; 958.517; 989.555;
2D 1027.506; 1156.618; 1272.692; 1310.654; 1341.728; 1600.819; 1890.936;
2D 2320.031; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001LAE: 823.408; 958.481; 989.518; 1027.49;
2D 1156.593; 1192.651; 1310.64; 1341.73; 1600.814; 1890.979; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
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DR ECO2DBASE; G036.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P08312; SYFA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(5.5-6.7)
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Escherichia coli(5-6)
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SWISS-2DPAGE (search AC)
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