Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

TBB5_MOUSE:

ID   TBB5_MOUSE; STANDARD; 2DG.
AC   P99024; P05218;
DT   01-SEP-1998, integrated into SWISS-2DPAGE (release 7).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Tubulin beta-5 chain;.
GN   Name=Tubb5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
MT   ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
IM   ISLETS_MOUSE, LIVER_MOUSE, MUSCLE_MOUSE, WAT_MOUSE.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano
RA   S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A.,
RA   Hochstrasser D.F.;
RT   ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of
RT   diabetes and obesity'';'';'';
RL   Proteomics 1(1):136-163(2001).
2D   -!- MASTER: ISLETS_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0018JQ=5.00/54284;
2D   -!-   PI/MW: SPOT 2D-0018K2=5.02/54284;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0018JQ: 1039.541; 1077.47; 1130.544;
2D         1143.575; 1159.56; 1229.555; 1245.548; 1287.659; 1620.836; 1631.802;
2D         1636.795; 1659.877; 1958.966; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-0018K2: 1028.493; 1039.598; 1077.535;
2D         1130.601; 1143.63; 1159.618; 1229.619; 1245.591; 1287.706; 1301.636;
2D         1620.868; 1631.825; 1636.848; 1659.887; 1822.912; 1958.993; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: LIVER_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0013GJ=4.97/49770;
2D   -!-   MAPPING: MICROSEQUENCING (MREIV) [1].
2D   -!- MASTER: MUSCLE_MOUSE;
2D   -!-   PI/MW: SPOT 2D-0016U7=4.94/53246;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0016U7: 971.479; 1039.602; 1077.514;
2D         1130.603; 1159.625; 1245.597; 1636.837; 1958.99; 2798.282; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: WAT_MOUSE;
2D   -!-   PI/MW: SPOT 2D-001B4W=5.02/49279;
2D   -!-   MAPPING: MATCHING WITH THE MOUSE LIVER MASTER GEL [1].
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DR   REPRODUCTION-2DPAGE; P99024; P99024.
DR   REPRODUCTION-2DPAGE; IPI00117352; IPI00117352.
DR   UniProtKB/Swiss-Prot; P99024; TBB5_MOUSE.
//

The following 2-D maps are available for this protein:

	Pancreatic islet cells	Liver	Gastrocnemius muscle
	White adipose tissue

SWISS-2DPAGE (search AC)