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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: THIE_ECOLI |
SWISS-2DPAGE: THIE_ECOLI
THIE_ECOLI:
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ID THIE_ECOLI; STANDARD; 2DG.
AC P30137;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 6.
DE RecName: Full=Thiamine-phosphate pyrophosphorylase; Short=TMP
DE pyrophosphorylase; Short=TMP-PPase; EC=2.5.1.3; AltName:
DE Full=Thiamine-phosphate synthase;.
GN Name=thiE; OrderedLocusNames=b3993, JW3957;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WU0=5.51/24964;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WU0: (R)SGLYPVVDSVQWIER(L),13-27;
2D (R)LFINDYWR(L),66-73; (R)LAIKHQAYGVHLGQEDLQATDLNAIR,74-99;
2D (K)HQAYGVHLGQEDLQATDLNAIR(A),78-99; (R)LADYPTVAIGGISLAR(A),156-171.
2D -!- MAPPING: Tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P30137; THIE_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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