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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: GLNA_ECOLI |
SWISS-2DPAGE: GLNA_ECOLI
GLNA_ECOLI:
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ID GLNA_ECOLI; STANDARD; 2DG.
AC P0A9C5; P06711;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 15.
DE RecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName:
DE Full=Glutamate--ammonia ligase;.
GN Name=glnA; OrderedLocusNames=b3870, JW3841;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4-5, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: OLIGOMER OF 12 SUBUNITS ARRANGED IN THE FORM OF TWO HEXAGON
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KPC=5.24/53915;
2D -!- PI/MW: SPOT 2D-000KPP=5.18/53809;
2D -!- PI/MW: SPOT 2D-000L4Q=5.39/36748;
2D -!- MAPPING: SPOTS 2D-000KPC, 2D-000KPP: GEL MATCHING [1] AND
2D IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]; SPOT 2D-000L4Q: AMINO ACID
2D COMPOSITION AND SEQUENCE TAG (SAEH) [3].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HLA=5.00/26823; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001HLA: 922.452; 1107.568; 1184.524;
2D 1200.499; 1579.716; 1636.754; 1717.847; 2004.902; 2310.093; 2495.221;
2D 2586.265; 3113.389; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WHR=5.30/53162;
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WHR: (K)RAEDYLR(S),110-116;
2D (K)GGYFPVPPVDSAQDIR(S),177-192; (K)AGGVFTDEAIDAYIALR(R),430-446.
2D -!- EXPRESSION: decrease after benzoic acid treatment [5].
2D -!- MAPPING: Tandem mass spectrometry [5].
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DR 2DBase-Ecoli; P0A9C5; GLNA_ECOLI.
DR ECO2DBASE; D049.9; 6TH EDITION.
DR ECO2DBASE; D050.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A9C5; GLNA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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