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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GLNA_ECOLI




SWISS-2DPAGE: GLNA_ECOLI



GLNA_ECOLI:

Nice View - a user-friendly view of this entry
ID   GLNA_ECOLI; STANDARD; 2DG.
AC   P0A9C5; P06711;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 15.
DE   RecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName:
DE   Full=Glutamate--ammonia ligase;.
GN   Name=glnA; OrderedLocusNames=b3870, JW3841;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: OLIGOMER OF 12 SUBUNITS ARRANGED IN THE FORM OF TWO HEXAGON 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KPC=5.24/53915;
2D   -!-   PI/MW: SPOT 2D-000KPP=5.18/53809;
2D   -!-   PI/MW: SPOT 2D-000L4Q=5.39/36748;
2D   -!-   MAPPING: SPOTS 2D-000KPC, 2D-000KPP: GEL MATCHING [1] AND
2D         IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]; SPOT 2D-000L4Q: AMINO ACID
2D         COMPOSITION AND SEQUENCE TAG (SAEH) [3].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HLA=5.00/26823; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HLA: 922.452; 1107.568; 1184.524;
2D         1200.499; 1579.716; 1636.754; 1717.847; 2004.902; 2310.093; 2495.221;
2D         2586.265; 3113.389; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WHR=5.30/53162;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WHR: (K)RAEDYLR(S),110-116;
2D         (K)GGYFPVPPVDSAQDIR(S),177-192; (K)AGGVFTDEAIDAYIALR(R),430-446.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [5].
2D   -!-   MAPPING: Tandem mass spectrometry [5].
CC   ---------------------------------------------------------------------------
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CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
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CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0A9C5; GLNA_ECOLI.
DR   ECO2DBASE; D049.9; 6TH EDITION.
DR   ECO2DBASE; D050.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A9C5; GLNA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server