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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GLNA_ECOLI




SWISS-2DPAGE:  GLNA_ECOLI


GLNA_ECOLI


General information about the entry
View entry in simple text format
Entry nameGLNA_ECOLI
Primary accession numberP0A9C5
Secondary accession number(s) P06711
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 4)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase;.
Gene nameName=glnA
OrderedLocusNames=b3870, JW3841
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: OLIGOMER OF 12 SUBUNITS ARRANGED IN THE FORM OF TWO HEXAGON
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.24; Mw=53915
pI=5.18; Mw=53809
pI=5.39; Mw=36748  [identification data]

MAPPING (identification):
SPOTS 2D-000KPC, 2D-000KPP: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2];
SPOT 2D-000L4Q: AMINO ACID COMPOSITION AND SEQUENCE TAG (SAEH) [3].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=5.00; Mw=26823  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.30; Mw=53162  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [5].

MAPPING (identification):
Tandem mass spectrometry [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A9C5; GLNA_ECOLI.
ECO2DBASED049.9; 6TH EDITION.
ECO2DBASED050.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0A9C5; GLNA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGLNA_ECOLI
Primary accession numberP0A9C5
Secondary accession number(s) P06711 Q2M8G7
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 59)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase;
Gene nameName=glnA
OrderedLocusNames=b3870, JW3841
Encoded onName=glnA; OrderedLocusNames=b3870, JW3841
KeywordsATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX05173; CAA28806.1; -; Genomic_DNA
EMBLM13746; AAA23879.1; -; Genomic_DNA
EMBLL19201; AAB03004.1; -; Genomic_DNA
EMBLU00096; AAC76867.1; -; Genomic_DNA
EMBLAP009048; BAE77439.1; -; Genomic_DNA
EMBLJ01618; AAA98066.1; -; Genomic_DNA
EMBLM10421; AAA23882.1; -; Genomic_DNA
EMBLK02176; AAA23880.1; -; Genomic_DNA
PIRS40815; AJECQ; .
RefSeqNP_418306.1; NC_000913.2; .
ProteinModelPortalP0A9C5; -; .
SMRP0A9C5; 2-469; .
IntActP0A9C5; 9; .
SWISS-2DPAGEP0A9C5; -; .
2DBase-EcoliP0A9C5; -; .
ECO2DBASED049.9; 6TH EDITION; .
ECO2DBASED050.0; 6TH EDITION; .
PRIDEP0A9C5; -; .
EnsemblBacteriaEBESCT00000004623; EBESCP00000004623; EBESCG00000003771; .
EnsemblBacteriaEBESCT00000004624; EBESCP00000004624; EBESCG00000003771; .
EnsemblBacteriaEBESCT00000004625; EBESCP00000004625; EBESCG00000003771; .
EnsemblBacteriaEBESCT00000004626; EBESCP00000004626; EBESCG00000003771; .
EnsemblBacteriaEBESCT00000017620; EBESCP00000016911; EBESCG00000016676; .
GeneID948370; -; .
GenomeReviewsAP009048_GR; JW3841; .
GenomeReviewsU00096_GR; b3870; .
KEGGecj:JW3841; -; .
KEGGeco:b3870; -; .
EchoBASEEB0378; -; .
EcoGeneEG10383; glnA; .
eggNOGCOG0174; -; .
GeneTreeEBGT00050000010403; -; .
HOGENOMHBG553755; -; .
OMAANAGQCE; -; .
ProtClustDBPRK09469; -; .
BioCycEcoCyc:GLUTAMINESYN-MONOMER; -; .
BioCycMetaCyc:GLUTAMINESYN-MONOMER; -; .
GenevestigatorP0A9C5; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004356; F:glutamate-ammonia ligase activity; IEA:EC; .
GOGO:0006542; P:glutamine biosynthetic process; IEA:InterPro; .
GOGO:0009399; P:nitrogen fixation; IEA:InterPro; .
InterProIPR008147; Gln_synt_beta; .
InterProIPR014746; Gln_synth/guanido_kin_cat_dom; .
InterProIPR008146; Gln_synth_cat_dom; .
InterProIPR004809; Gln_synth_I; .
InterProIPR001637; Gln_synth_I_adenylation_site; .
Gene3DG3DSA:3.30.590.10; ATP-gua_Ptrans; 1; .
Gene3DG3DSA:3.10.20.70; G3DSA:3.10.20.70; 1; .
PfamPF00120; Gln-synt_C; 1; .
PfamPF03951; Gln-synt_N; 1; .
SUPFAMSSF54368; Gln_synt_beta; 1; .
TIGRFAMsTIGR00653; GlnA; 1; .
PROSITEPS00180; GLNA_1; 1; .
PROSITEPS00182; GLNA_ADENYLATION; 1; .
PROSITEPS00181; GLNA_ATP; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server