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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: IDH_ECOLI




SWISS-2DPAGE: IDH_ECOLI



IDH_ECOLI:

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ID   IDH_ECOLI; STANDARD; 2DG.
AC   P08200;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42;
DE   AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName:
DE   Full=Oxalosuccinate decarboxylase;.
GN   Name=icd; Synonyms=icdA, icdE; OrderedLocusNames=b1136, JW1122;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=81069671; PubMed=7002901; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Phillips T.A., Bloch P.L., Neidhardt F.C.;
RT   ''''''Protein identifications on O'Farrell two-dimensional gels:
RT   locations of 55 additional Escherichia coli proteins'';'';'';
RL   J. Bacteriol. 144(1):1024-1033(1980).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: MONOMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KVS=5.02/46142;
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN
2D         [2] AND PHILLIPS [3].
2D   -!- MASTER: ECOLI4-5;
2D   -!-   PI/MW: SPOT 2D-001HM1=4.70/22389; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001HM1: 1008.518; 1030.514; 1206.644;
2D         1470.789; 1630.906; 1646.858; 1788.869; 2086.112; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-0014YW=5.15/43958;
2D   -!-   PEPTIDE MASSES: SPOT 2D-0014YW: 1030.419; 1124.567; 1206.55;
2D         1373.612; 1662.74; 2085.952; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001KVT=5.00/74518;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001KVT: 1008.45; 1030.433; 1124.616;
2D         1206.587; 1357.692; 1470.752; 1484.719; 1553.721; 1630.906; 1646.851;
2D         1989.021; 2086.181; 2202.183; 2304.309; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WKP=5.11/44335;
2D   -!-   PI/MW: SPOT 2D-001WKS=5.15/44053;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WKP: 906.44; 1030.49; 1124.65; 1139.61;
2D         1206.65; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WKS: 1124.73; 1139.57; 1206.66; 1357.68;
2D         1373.63; 1646.87; 1662.91; 1674.96; 2045.85; 2085.91; TRYPSIN.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [5].
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P08200; IDH_ECOLI.
DR   ECO2DBASE; C043.8; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P08200; IDH_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4-5)
ECOLI4-5
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
 


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server