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It will be discontinued on 31-May-2024.

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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P21550



General information about the entry
View entry in simple text format
Entry nameENOB_MOUSE
Primary accession numberP21550
integrated into SWISS-2DPAGE on June 30, 1999 (release 10)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Beta-enolase; EC=; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;.
Gene nameName=Eno3
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
  map experimental info
  protein estimated location

pI=6.05; Mw=47683  [identification data]
pI=6.25; Mw=47420  [identification data]
pI=6.17; Mw=47160  [identification data]
pI=6.33; Mw=46771  [identification data]
pI=6.38; Mw=46642  [identification data]
pI=6.68; Mw=45750  [identification data]
pI=6.44; Mw=46514  [identification data]
pI=6.52; Mw=46003  [identification data]
pI=6.17; Mw=45876  [identification data]
pI=6.03; Mw=44875  [identification data]
pI=6.38; Mw=41535  [identification data]
pI=6.45; Mw=40985  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
UniProtKB/Swiss-ProtP21550; ENOB_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameENOB_MOUSE
Primary accession numberP21550
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 121)
Name and origin of the protein
DescriptionRecName: Full=Beta-enolase; EC=; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;
Gene nameName=Eno3
Encoded onName=Eno3; Synonyms=Eno-3
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Reference proteome.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLX61600; CAA43797.1; -; Genomic_DNA
EMBLX57747; CAA40913.1; -; mRNA
EMBLX62667; CAA44540.1; -; mRNA
EMBLAK002485; BAB22137.1; -; mRNA
EMBLBC013460; AAH13460.1; -; mRNA
EMBLM20745; AAA37554.1; -; mRNA
IPIIPI00228548; -; .
PIRS17109; NOMSB; .
RefSeqNP_001129534.1; NM_001136062.1; .
RefSeqNP_031959.1; NM_007933.2; .
UniGeneMm.251322; -; .
ProteinModelPortalP21550; -; .
SMRP21550; 1-434; .
IntActP21550; 1; .
STRINGP21550; -; .
PhosphoSiteP21550; -; .
SWISS-2DPAGEP21550; -; .
UCD-2DPAGEP21550; -; .
PRIDEP21550; -; .
EnsemblENSMUST00000072841; ENSMUSP00000072620; ENSMUSG00000060600; .
EnsemblENSMUST00000108548; ENSMUSP00000104188; ENSMUSG00000060600; .
EnsemblENSMUST00000170716; ENSMUSP00000128714; ENSMUSG00000060600; .
GeneID13808; -; .
KEGGmmu:13808; -; .
CTD2027; -; .
MGIMGI:95395; Eno3; .
eggNOGroNOG10078; -; .
HOGENOMHBG726599; -; .
HOVERGENHBG000067; -; .
InParanoidP21550; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP21550; -; .
BRENDA4.2.1.11; 3474; .
NextBio284592; -; .
ArrayExpressP21550; -; .
BgeeP21550; -; .
CleanExMM_ENO3; -; .
GenevestigatorP21550; -; .
GermOnlineENSMUSG00000060600; Mus musculus; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IEA:EC; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
TIGRFAMsTIGR01060; Eno; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server