Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P30050:

ID   RL12_HUMAN; STANDARD; 2DG.
AC   P30050;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=60S ribosomal protein L12;.
GN   Name=RPL12;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, NUCLEOLI_HELA_1D_HUMAN.
IM   CEC_HUMAN, NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001VA5=20822;
1D   -!-   MW: BAND 1D-001VA6=20347;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001VA5: (R)QAQIEVVPSASALIIK(A),68-83; (K)HSGNITFDEIVNIAR(Q),100-114.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001VA6: (R)CTGGEVGATSALAPK(I),17-31;
1D         (K)IGPLGLSPK(K),32-40; (R)QAQIEVVPSASALIIK(A),68-83; (K)HSGNITFDEIVNIAR(Q),100-114.
1D   -!-   MAPPING: Tandem mass spectrometry [2].
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000V17=5.31/11038;
2D   -!-   MAPPING: MICROSEQUENCING [1].
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DR   UniProtKB/Swiss-Prot; P30050; RL12_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal epithelia cells	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)