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Protein list for SWISS-2DPAGE HL60_HUMAN 
Promyelocytic leukemia cells  {Homo sapiens (Human)}
tissue: Promyelocytic leukemia


(map ID: a3e72dc4-f64a-4d51-b636-bf88c565d0f4)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for hl60_human   26 identified spots / 17 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-000YZK
2D-000Z0A
2D-000Z0G
5.26
5.16
5.08
41'605
41'070
41'177
{Gm}
{Gm}
{Gm}
-
-
-
1, 2
1, 2
1, 2
ATP5D RecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor; P30049
(ATPD_HUMAN)
2D-00101J
4.40
12'724
{Gm}
-
1, 2
CALR RecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor; P27797
(CALR_HUMAN)
2D-000YMC
4.33
57'239
{Gm}
-
1, 2
COX5A RecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor; P20674
(COX5A_HUMAN)
2D-00104G
4.90
11'355
{Gm}
-
1, 2
HSPA9 RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor; P38646
(GRP75_HUMAN)
2D-000YIK
2D-000YIG
2D-000YI8
5.18
5.23
5.28
67'055
67'250
67'250
{Gm}
{Gm}
{Gm}
-
-
-
1, 2
1, 2
1, 2
HSPD1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor; P10809
(CH60_HUMAN)
2D-000YMF
5.25
57'962
{Gm}
-
1, 2
LGALS1 RecName: Full=Galectin-1; Short=Gal-1; AltName: Full=14 kDa laminin-binding protein; Short=HLBP14; AltName: Full=14 kDa lectin; AltName: Full=Beta-galactoside-binding lectin L-14-I; AltName: Full=Galaptin; AltName: Full=HBL; AltName: Full=HPL; AltName: Full=Lactose-binding lectin 1; AltName: Full=Lectin galactoside-binding soluble 1; AltName: Full=Putative MAPK-activating protein PM12; AltName: Full=S-Lac lectin 1; P09382
(LEG1_HUMAN)
2D-00104W
5.01
11'094
{Co}
Expression
1, 2, 3
P4HB RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor; P07237
(PDIA1_HUMAN)
2D-000YNF
4.73
56'100
{Gm}
-
1, 2
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-000YPE
2D-000YPU
5.57
5.68
53'351
52'818
{Gm}
{Gm}
-
-
1, 2
1, 2
PPIA RecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A; EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin A-binding protein; AltName: Full=Rotamase A; P62937
(PPIA_HUMAN)
2D-001007
2D-00100I
2D-00100F
6.70
7.08
6.44
13'227
13'159
13'193
{Gm}
{Gm}
{Gm}
-
-
-
1, 2
1, 2
1, 2
PRDX6 RecName: Full=Peroxiredoxin-6; EC=1.11.1.15; AltName: Full=1-Cys peroxiredoxin; Short=1-Cys PRX; AltName: Full=24 kDa protein; AltName: Full=Acidic calcium-independent phospholipase A2; Short=aiPLA2; EC=3.1.1.-; AltName: Full=Antioxidant protein 2; AltName: Full=Liver 2D page spot 40; AltName: Full=Non-selenium glutathione peroxidase; Short=NSGPx; EC=1.11.1.9; AltName: Full=Red blood cells page spot 12; P30041
(PRDX6_HUMAN)
2D-000ZM3
6.35
23'368
{Gm}
-
1, 2
RPLP2 RecName: Full=60S acidic ribosomal protein P2; AltName: Full=Renal carcinoma antigen NY-REN-44; P05387
(RLA2_HUMAN)
2D-00100Y
4.28
12'956
{Gm}
-
1, 2
SOD1 RecName: Full=Superoxide dismutase [Cu-Zn]; EC=1.15.1.1; AltName: Full=Superoxide dismutase 1; Short=hSod1; P00441
(SODC_HUMAN)
2D-000ZXR
5.60
14'745
{Gm}
-
1, 2
SOD2 RecName: Full=Superoxide dismutase [Mn], mitochondrial; EC=1.15.1.1; Flags: Precursor; P04179
(SODM_HUMAN)
2D-000ZSJ
2D-000ZSL
6.94
7.32
18'707
18'707
{Gm}
{Gm}
-
-
1, 2
1, 2
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P60174
(TPIS_HUMAN)
2D-000ZMG
2D-000ZMM
6.55
6.84
23'187
23'068
{Gm}
{Gm}
-
-
1, 2
1, 2
TXN RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor; Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP; P10599
(THIO_HUMAN)
2D-00105M
4.89
10'727
{Gm}
-
1, 2
UBC RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor; P0CG48
(UBC_HUMAN)
2D-00109F
6.90
8'565
{Gm}
-
1, 2
Mapping legends: { Co } => 'Comigration', { Gm } => 'Gel matching'



Cited references

# PubMed/Medline Authors Location
[1] - Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.; Submitted (JUL-1997) to SWISS-2DPAGE.
[2] 8740185 Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.; Electrophoresis 17(1):600-606(1996).
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
[3] 2910856 Couraud P.O., Casentini-Borocz D., Bringman T.S., Griffith J., McGrogan M., Nedwin G.E.; J. Biol. Chem. 264(1):1310-1316(1989).
''''''Molecular cloning, characterization, and expression of a human 14-kDa lectin'';'';'';


Gel experimental information

Information Type Data
Map name HL60_HUMAN {Promyelocytic leukemia cells}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
species Homo sapiens (Human) / TaxID:9606
Tissue (Swiss-Prot definition) Promyelocytic leukemia
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 3164
Number of identified spots 26
Number of identified proteins 17
Reference Bibliographic reference
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server