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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P01024




SWISS-2DPAGE:  P01024


P01024


General information about the entry
View entry in simple text format
Entry nameCO3_HUMAN
Primary accession numberP01024
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Complement C3; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Contains: RecName: Full=Complement C3 beta chain; Contains: RecName: Full=Complement C3 alpha chain; Contains: RecName: Full=C3a anaphylatoxin; Contains: RecName: Full=Complement C3b alpha' chain; Contains: RecName: Full=Complement C3c alpha' chain fragment 1; Contains: RecName: Full=Complement C3dg fragment; Contains: RecName: Full=Complement C3g fragment; Contains: RecName: Full=Complement C3d fragment; Contains: RecName: Full=Complement C3f fragment; Contains: RecName: Full=Complement C3c alpha' chain fragment 2; Flags: Precursor;.
Gene nameName=C3
Synonyms=CPAMD1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.
''''''Plasma protein map: an update by microsequencing'';'';''
Electrophoresis 13(1):707-714(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: ALPHA AND BETA CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=5.91; Mw=104427
pI=5.99; Mw=103408
pI=6.14; Mw=102400
pI=6.07; Mw=102400
pI=6.21; Mw=101899

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=5.69; Mw=106396
pI=5.77; Mw=104612
pI=5.85; Mw=102280
pI=5.92; Mw=101705
pI=6.00; Mw=100000
pI=6.13; Mw=98894
pI=6.26; Mw=98528
pI=6.39; Mw=97800

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=6.81; Mw=70675
pI=6.50; Mw=71216
pI=6.66; Mw=70675
pI=6.41; Mw=71488
pI=6.98; Mw=70675
pI=4.84; Mw=40915

NORMAL LEVEL:
550-1200 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING LUPUS ERYTHEMATOSUS AND MEMBRANOPROLIFERATIVE GLOMERULONEPHRITIS; INCREASED DURING ACUTE INFLAMMATORY REACTIONS.

MAPPING (identification):
MICROSEQUENCING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP01024; P01024.
DOSAC-COBS-2DPAGEP01024; CO3_HUMAN.
Siena-2DPAGEP01024; CO3_HUMAN.
UniProtKB/Swiss-ProtP01024; CO3_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCO3_HUMAN
Primary accession numberP01024
Secondary accession number(s) A7E236
Sequence was last modified on December 12, 2006 (version 2)
Annotations were last modified on October 19, 2011 (version 153)
Name and origin of the protein
DescriptionRecName: Full=Complement C3; AltName: Full=C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Contains: RecName: Full=Complement C3 beta chain; Contains: RecName: Full=Complement C3 alpha chain; Contains: RecName: Full=C3a anaphylatoxin; Contains: RecName: Full=Complement C3b alpha' chain; Contains: RecName: Full=Complement C3c alpha' chain fragment 1; Contains: RecName: Full=Complement C3dg fragment; Contains: RecName: Full=Complement C3g fragment; Contains: RecName: Full=Complement C3d fragment; Contains: RecName: Full=Complement C3f fragment; Contains: RecName: Full=Complement C3c alpha' chain fragment 2; Flags: Precursor;
Gene nameName=C3
Synonyms=CPAMD1
Encoded onName=C3; Synonyms=CPAMD1
Keywords3D-structure; Age-related macular degeneration; Cleavage on pair of basic residues; Complement alternate pathway; Complement pathway; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Hemolytic uremic syndrome; Immunity; Inflammatory response; Innate immunity; Phosphoprotein; Polymorphism; Reference proteome; Secreted; Signal; Thioester bond.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK02765; AAA85332.1; -; mRNA
EMBLAY513239; AAR89906.1; -; Genomic_DNA
EMBLCH471139; EAW69071.1; -; Genomic_DNA
EMBLBC150179; AAI50180.1; -; mRNA
EMBLBC150200; AAI50201.1; -; mRNA
IPIIPI00783987; -; .
PIRA94065; C3HU; .
RefSeqNP_000055.2; NM_000064.2; .
UniGeneHs.529053; -; .
PDB1C3D; X-ray; 1.80 A; A=996-1287
PDB1GHQ; X-ray; 2.04 A; A=996-1300
PDB1W2S; X-ray; -; A=996-1299
PDB2A73; X-ray; 3.30 A; A=23-665
B=673-1663PDB; 2A74; X-ray; 2.40 A
A/D=23-665B/E=749-936; C/F=1321-1663; PDB; 2GOX
X-ray2.20 A; A/C=996-1287; PDB; 2HR0
X-ray2.26 A; A=23-667; B=749-1663; PDB
2I07X-ray; 4.00 A; A=23-667; B=749-1663
PDB2ICE; X-ray; 3.10 A; A/D=23-664
B/E=749-954C/F=1321-1663; PDB; 2ICF; X-ray
4.10 AA=23-664; B=749-1663; PDB; 2NOJ
X-ray2.70 A; A/C/E/G=996-1287; PDB; 2QKI
X-ray2.40 A; A/D=23-665; B/E=749-936; C/F=1321-1663
PDB2WII; X-ray; 2.70 A; A=23-667
B=749-1663PDB; 2WIN; X-ray; 3.90 A
A/C/E/G=23-667B/D/F/H=749-1663; PDB; 2WY7; X-ray
1.70 AA=996-1303; PDB; 2WY8; X-ray
1.70 AA=996-1303; PDB; 2XQW; X-ray
2.31 AA/B=996-1287; PDB; 2XWB; X-ray
3.49 AA/C=23-664; B/D=752-1663; PDB; 2XWJ
X-ray4.00 A; A/C/E/G=23-667; B/D/F/H=749-1663; PDB
3D5RX-ray; 2.10 A; A/B=996-1287; PDB
3D5SX-ray; 2.30 A; A/B=996-1287; PDB
3G6JX-ray; 3.10 A; A/C=23-666; B/D=749-1663
PDB3L3O; X-ray; 3.40 A; A/D=23-667
B/E=749-954C/F=1321-1663; PDB; 3L5N; X-ray
7.54 AA=23-667; B=749-1663; PDB; 3NMS
X-ray4.10 A; A=23-667; B=749-954; C=1321-1663
PDB3OED; X-ray; 3.16 A; A/B=996-1303
PDB3OHX; X-ray; 3.50 A; A/D=23-667
B/E=749-954C/F=1321-1663; PDB; 3OXU; X-ray
2.10 AA/B/C=996-1303; PDBsum; 1C3D; -
PDBsum1GHQ; -; .
PDBsum1W2S; -; .
PDBsum2A73; -; .
PDBsum2A74; -; .
PDBsum2GOX; -; .
PDBsum2HR0; -; .
PDBsum2I07; -; .
PDBsum2ICE; -; .
PDBsum2ICF; -; .
PDBsum2NOJ; -; .
PDBsum2QKI; -; .
PDBsum2WII; -; .
PDBsum2WIN; -; .
PDBsum2WY7; -; .
PDBsum2WY8; -; .
PDBsum2XQW; -; .
PDBsum2XWB; -; .
PDBsum2XWJ; -; .
PDBsum3D5R; -; .
PDBsum3D5S; -; .
PDBsum3G6J; -; .
PDBsum3L3O; -; .
PDBsum3L5N; -; .
PDBsum3NMS; -; .
PDBsum3OED; -; .
PDBsum3OHX; -; .
PDBsum3OXU; -; .
ProteinModelPortalP01024; -; .
SMRP01024; 23-664; 673-1663; .
DIPDIP-35180N; -; .
IntActP01024; 4; .
STRINGP01024; -; .
MEROPSI39.950; -; .
GlycoSuiteDBP01024; -; .
PhosphoSiteP01024; -; .
SWISS-2DPAGEP01024; -; .
Cornea-2DPAGEP01024; -; .
DOSAC-COBS-2DPAGEP01024; -; .
Siena-2DPAGEP01024; -; .
PeptideAtlasP01024; -; .
PRIDEP01024; -; .
EnsemblENST00000245907; ENSP00000245907; ENSG00000125730; .
GeneID718; -; .
KEGGhsa:718; -; .
UCSCuc002mfm.1; human; .
CTD718; -; .
GeneCardsGC19M006440; -; .
H-InvDBHIX0020036; -; .
HGNCHGNC:1318; C3; .
HPACAB004209; -; .
HPAHPA003563; -; .
HPAHPA020432; -; .
MIM120700; gene; .
MIM611378; phenotype; .
MIM612925; phenotype; .
MIM613779; phenotype; .
neXtProtNX_P01024; -; .
Orphanet279; Age-related macular degeneration; .
Orphanet93575; Atypical hemolytic uremic syndrome with C3 anomaly; .
PharmGKBPA25897; -; .
eggNOGprNOG14935; -; .
HOGENOMHBG755386; -; .
HOVERGENHBG005110; -; .
InParanoidP01024; -; .
OMAVTVHDFP; -; .
OrthoDBEOG41G337; -; .
PhylomeDBP01024; -; .
ReactomeREACT_14797; Signaling by GPCR; .
ReactomeREACT_6900; Immune System; .
NextBio2922; -; .
PMAP-CutDBP01024; -; .
ArrayExpressP01024; -; .
BgeeP01024; -; .
CleanExHS_C3; -; .
GenevestigatorP01024; -; .
GermOnlineENSG00000125730; Homo sapiens; .
GOGO:0005615; C:extracellular space; IEA:InterPro; .
GOGO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro; .
GOGO:0005102; F:receptor binding; TAS:ProtInc; .
GOGO:0006957; P:complement activation; alternative pathway; TAS:Reactome
GOGO:0006958; P:complement activation; classical pathway; IEA:UniProtKB-KW
GOGO:0007186; P:G-protein coupled receptor protein signaling pathway; TAS:ProtInc; .
GOGO:0006954; P:inflammatory response; IEA:UniProtKB-KW; .
GOGO:0010575; P:positive regulation vascular endothelial growth factor production; IDA:BHF-UCL; .
InterProIPR009048; A-macroglobulin_rcpt-bd; .
InterProIPR011626; A2M_comp; .
InterProIPR002890; A2M_N; .
InterProIPR011625; A2M_N_2; .
InterProIPR000020; Anaphylatoxin/fibulin; .
InterProIPR018081; Anaphylatoxin_; .
InterProIPR001840; Anaphylatoxn; .
InterProIPR001599; Macroglobln_a2; .
InterProIPR019742; MacrogloblnA2_CS; .
InterProIPR019565; MacrogloblnA2_thiol-ester-bond; .
InterProIPR001134; Netrin_domain; .
InterProIPR018933; Netrin_module_non-TIMP; .
InterProIPR008930; Terpenoid_cyclase/PrenylTrfase; .
InterProIPR008993; TIMP-like_OB-fold; .
Gene3DG3DSA:2.60.40.690; A-macroglobulin_rcpt-bd; 1; .
Gene3DG3DSA:1.20.91.20; Anaphylatoxin; 1; .
PfamPF00207; A2M; 1; .
PfamPF07678; A2M_comp; 1; .
PfamPF01835; A2M_N; 1; .
PfamPF07703; A2M_N_2; 1; .
PfamPF07677; A2M_recep; 1; .
PfamPF01821; ANATO; 1; .
PfamPF01759; NTR; 1; .
PfamPF10569; Thiol-ester_cl; 1; .
PRINTSPR00004; ANAPHYLATOXN; .
SMARTSM00104; ANATO; 1; .
SMARTSM00643; C345C; 1; .
SUPFAMSSF49410; AM_receptor_bind; 1; .
SUPFAMSSF47686; Anaphylatoxin; 1; .
SUPFAMSSF48239; Terp_cyc_toroid; 1; .
SUPFAMSSF50242; TIMP_like; 1; .
PROSITEPS00477; ALPHA_2_MACROGLOBULIN; 1; .
PROSITEPS01177; ANAPHYLATOXIN_1; 1; .
PROSITEPS01178; ANAPHYLATOXIN_2; 1; .
PROSITEPS50189; NTR; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server