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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P20152




SWISS-2DPAGE:  P20152


P20152


General information about the entry
View entry in simple text format
Entry nameVIME_MOUSE
Primary accession numberP20152
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;.
Gene nameName=Vim
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=4.85; Mw=38681  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=5.11; Mw=59488  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=5.16; Mw=59607  [identification data]
pI=5.13; Mw=53059

MAPPING (identification):
SPOT 2D-00138C: Peptide mass fingerprinting [2];
SPOT 2D-0013E4: MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=5.14; Mw=55496

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].



NUCLEI_LIVER_MOUSE {Soluble nuclear proteins and matrix from liver tissue}
Mus musculus (Mouse)
Tissue: Liver
NUCLEI_LIVER_MOUSE
  map experimental info
  protein estimated location
 
NUCLEI_LIVER_MOUSE

MAP LOCATIONS:
pI=5.09; Mw=55195  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=5.12; Mw=53967  [identification data]
pI=4.92; Mw=39581  [identification data]

MAPPING (identification):
SPOT 2D-001B0W: MATCHING WITH THE MOUSE LIVER MASTER GEL [2];
SPOT 2D-001BI9: Peptide mass fingerprinting [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEIPI00227299; IPI00227299.
UCD-2DPAGEP20152; VIME_MOUSE.
UniProtKB/Swiss-ProtP20152; VIME_MOUSE.
World-2DPAGE RepositoryP20152; VIME_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameVIME_MOUSE
Primary accession numberP20152
Secondary accession number(s) O08704 Q8CCH1
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 127)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;
Gene nameName=Vim
Encoded onName=Vim
KeywordsAcetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Intermediate filament; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM24849; AAA40555.1; -; mRNA
EMBLX56397; CAA39807.1; -; mRNA
EMBLM26251; AAA40556.1; -; mRNA
EMBLZ22526; CAA80251.1; -; Genomic_DNA
EMBLX51438; CAA35803.1; -; mRNA
EMBLY07738; CAA69019.1; -; Genomic_DNA
EMBLAK033175; BAC28181.1; -; mRNA
EMBLD50805; BAA19834.1; -; Genomic_DNA
IPIIPI00227299; -; .
PIRA43803; A43803; .
RefSeqNP_035831.2; NM_011701.4; .
UniGeneMm.268000; -; .
ProteinModelPortalP20152; -; .
SMRP20152; 101-138; 144-248; 263-406
DIPDIP-188N; -; .
IntActP20152; 7; .
MINTMINT-1202726; -; .
STRINGP20152; -; .
PhosphoSiteP20152; -; .
SWISS-2DPAGEP20152; -; .
REPRODUCTION-2DPAGEIPI00227299; -; .
UCD-2DPAGEP20152; -; .
PRIDEP20152; -; .
EnsemblENSMUST00000028062; ENSMUSP00000028062; ENSMUSG00000026728; .
GeneID22352; -; .
KEGGmmu:22352; -; .
CTD7431; -; .
MGIMGI:98932; Vim; .
HOGENOMHBG715391; -; .
HOVERGENHBG013015; -; .
InParanoidP20152; -; .
OMAANRTNEK; -; .
OrthoDBEOG4GHZPD; -; .
PhylomeDBP20152; -; .
NextBio302643; -; .
ArrayExpressP20152; -; .
BgeeP20152; -; .
CleanExMM_VIM; -; .
GenevestigatorP20152; -; .
GermOnlineENSMUSG00000026728; Mus musculus; .
GOGO:0031252; C:cell leading edge; IDA:MGI; .
GOGO:0045098; C:type III intermediate filament; TAS:MGI; .
GOGO:0005212; F:structural constituent of eye lens; IDA:MGI; .
GOGO:0014002; P:astrocyte development; IGI:MGI; .
GOGO:0060020; P:Bergmann glial cell differentiation; IMP:MGI; .
GOGO:0045109; P:intermediate filament organization; IGI:MGI; .
GOGO:0070307; P:lens fiber cell development; IDA:MGI; .
GOGO:0010977; P:negative regulation of neuron projection development; IGI:MGI; .
InterProIPR016044; F; .
InterProIPR001664; IF; .
InterProIPR006821; Intermed_filament_DNA-bd; .
InterProIPR018039; Intermediate_filament_CS; .
PANTHERPTHR23239; IF; 1; .
PfamPF00038; Filament; 1; .
PfamPF04732; Filament_head; 1; .
PROSITEPS00226; IF; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server