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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P99024




SWISS-2DPAGE:  P99024


P99024


General information about the entry
View entry in simple text format
Entry nameTBB5_MOUSE
Primary accession numberP99024
Secondary accession number(s) P05218
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Tubulin beta-5 chain;.
Gene nameName=Tubb5
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=5.00; Mw=54284  [identification data]
pI=5.02; Mw=54284  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=4.97; Mw=49770

MAPPING (identification):
MICROSEQUENCING (MREIV) [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.94; Mw=53246  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=5.02; Mw=49279

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP99024; P99024.
REPRODUCTION-2DPAGEIPI00117352; IPI00117352.
UniProtKB/Swiss-ProtP99024; TBB5_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTBB5_MOUSE
Primary accession numberP99024
Secondary accession number(s) B1B178 P05218 Q3TFB6 Q3THH9 Q3TIL1 Q3UAV4 Q3UF52 Q8WUC1 Q9CY33
Sequence was last modified on August 13, 1987 (version 1)
Annotations were last modified on October 19, 2011 (version 80)
Name and origin of the protein
DescriptionRecName: Full=Tubulin beta-5 chain;
Gene nameName=Tubb5
Encoded onName=Tubb5
KeywordsAcetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Isopeptide bond; Methylation; Microtubule; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX04663; CAA28369.1; -; mRNA
EMBLAK010960; BAB27292.1; -; mRNA
EMBLAK011263; BAB27504.1; -; mRNA
EMBLAK051164; BAC34541.1; -; mRNA
EMBLAK051393; BAC34623.1; -; mRNA
EMBLAK083327; BAC38866.1; -; mRNA
EMBLAK146567; BAE27265.1; -; mRNA
EMBLAK148978; BAE28709.1; -; mRNA
EMBLAK151215; BAE30210.1; -; mRNA
EMBLAK151670; BAE30596.1; -; mRNA
EMBLAK160225; BAE35700.1; -; mRNA
EMBLAK161575; BAE36471.1; -; mRNA
EMBLAK165249; BAE38103.1; -; mRNA
EMBLAK167691; BAE39738.1; -; mRNA
EMBLAK167779; BAE39811.1; -; mRNA
EMBLAK167808; BAE39835.1; -; mRNA
EMBLAK167926; BAE39931.1; -; mRNA
EMBLAK168271; BAE40217.1; -; mRNA
EMBLAK169123; BAE40903.1; -; mRNA
EMBLAK169210; BAE40982.1; -; mRNA
EMBLAK169295; BAE41051.1; -; mRNA
EMBLCR974451; CAO77890.1; -; Genomic_DNA
EMBLBC003825; AAH03825.1; -; mRNA
EMBLM28732; AAA40510.1; -; mRNA
IPIIPI00117352; -; .
PIRE25437; E25437; .
RefSeqNP_035785.1; NM_011655.5; .
UniGeneMm.273538; -; .
UniGeneMm.472638; -; .
ProteinModelPortalP99024; -; .
SMRP99024; 2-427; .
IntActP99024; 19; .
STRINGP99024; -; .
PhosphoSiteP99024; -; .
SWISS-2DPAGEP99024; -; .
REPRODUCTION-2DPAGEIPI00117352; -; .
REPRODUCTION-2DPAGEP99024; -; .
PRIDEP99024; -; .
EnsemblENSMUST00000001566; ENSMUSP00000001566; ENSMUSG00000001525; .
GeneID22154; -; .
KEGGmmu:22154; -; .
CTD22154; -; .
MGIMGI:107812; Tubb5; .
eggNOGroNOG14625; -; .
HOVERGENHBG000089; -; .
InParanoidP99024; -; .
OMARYQGEND; -; .
OrthoDBEOG4DFPNJ; -; .
PhylomeDBP99024; -; .
ReactomeREACT_17056; Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding; .
ReactomeREACT_93132; Metabolism of proteins; .
NextBio302074; -; .
ArrayExpressP99024; -; .
BgeeP99024; -; .
CleanExMM_TUBB5; -; .
GenevestigatorP99024; -; .
GermOnlineENSMUSG00000001525; Mus musculus; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005874; C:microtubule; IDA:MGI; .
GOGO:0045298; C:tubulin complex; NAS:UniProtKB; .
GOGO:0005525; F:GTP binding; NAS:UniProtKB; .
GOGO:0003924; F:GTPase activity; IEA:InterPro; .
GOGO:0005200; F:structural constituent of cytoskeleton; IDA:MGI; .
GOGO:0007018; P:microtubule-based movement; IEA:InterPro; .
GOGO:0006457; P:protein folding; TAS:Reactome; .
GOGO:0051258; P:protein polymerization; IEA:InterPro; .
GOGO:0051225; P:spindle assembly; IDA:MGI; .
InterProIPR013838; Beta-tubulin_BS; .
InterProIPR002453; Beta_tubulin; .
InterProIPR008280; Tub_FtsZ_C; .
InterProIPR000217; Tubulin; .
InterProIPR018316; Tubulin/FtsZ_2-layer-sand-dom; .
InterProIPR023123; Tubulin_C; .
InterProIPR017975; Tubulin_CS; .
InterProIPR003008; Tubulin_FtsZ_GTPase; .
Gene3DG3DSA:3.30.1330.20; Tubulin/FtsZ_2-layer-sand-dom; 1; .
Gene3DG3DSA:1.10.287.600; Tubulin_C; 1; .
Gene3DG3DSA:3.40.50.1440; Tubulin_FtsZ; 1; .
PANTHERPTHR11588; Tubulin; 1; .
PfamPF00091; Tubulin; 1; .
PfamPF03953; Tubulin_C; 1; .
PRINTSPR01163; BETATUBULIN; .
PRINTSPR01161; TUBULIN; .
SMARTSM00864; Tubulin; 1; .
SMARTSM00865; Tubulin_C; 1; .
SUPFAMSSF55307; Tub_FtsZ_C; 1; .
SUPFAMSSF52490; Tubulin_FtsZ; 1; .
PROSITEPS00227; TUBULIN; 1; .
PROSITEPS00228; TUBULIN_B_AUTOREG; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server