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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: Q05920




SWISS-2DPAGE:  Q05920


Q05920


General information about the entry
View entry in simple text format
Entry namePYC_MOUSE
Primary accession numberQ05920
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Synonyms=Pcx
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=6.13; Mw=106385
pI=6.01; Mw=105676
pI=6.10; Mw=105676
pI=6.15; Mw=104971
pI=6.06; Mw=105676

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.16; Mw=121389
pI=6.10; Mw=121389
pI=6.13; Mw=121389
pI=6.21; Mw=120000  [identification data]

MAPPING (identification):
MATCHING WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1], AND MASS FINGERPRINTING [2].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=6.21; Mw=118405  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=6.04; Mw=124588
pI=6.08; Mw=123040
pI=6.11; Mw=121510

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEQ05920; Q05920.
UniProtKB/Swiss-ProtQ05920; PYC_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePYC_MOUSE
Primary accession numberQ05920
Sequence was last modified on February 1, 1994 (version 1)
Annotations were last modified on October 19, 2011 (version 106)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Synonyms=Pcx
Encoded onName=Pc; Synonyms=Pcx
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL09192; AAA39737.1; -; mRNA
EMBLBC055030; AAH55030.1; -; mRNA
IPIIPI00943457; -; .
PIRA47255; A47255; .
UniGeneMm.1845; -; .
ProteinModelPortalQ05920; -; .
SMRQ05920; 35-490; 494-1178; .
IntActQ05920; 1; .
STRINGQ05920; -; .
PhosphoSiteQ05920; -; .
SWISS-2DPAGEQ05920; -; .
REPRODUCTION-2DPAGEQ05920; -; .
PRIDEQ05920; -; .
MGIMGI:97520; Pcx; .
eggNOGroNOG05375; -; .
HOVERGENHBG008340; -; .
InParanoidQ05920; -; .
OrthoDBEOG4ZCT3P; -; .
NextBio294388; -; .
ArrayExpressQ05920; -; .
BgeeQ05920; -; .
CleanExMM_PCX; -; .
GenevestigatorQ05920; -; .
GermOnlineENSMUSG00000024892; Mus musculus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IEA:EC; .
GOGO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS50991; PYR_CT; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server