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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: Q16822




SWISS-2DPAGE:  Q16822


Q16822


General information about the entry
View entry in simple text format
Entry namePCKGM_HUMAN
Primary accession numberQ16822
integrated into SWISS-2DPAGE on January 15, 1999 (release 9)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; Short=PEPCK-M; EC=4.1.1.32; AltName: Full=Phosphoenolpyruvate carboxylase; Flags: Precursor;.
Gene nameName=PCK2
Synonyms=PEPCK2
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=6.63; Mw=68813  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtQ16822; PCKGM_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePCKGM_HUMAN
Primary accession numberQ16822
Secondary accession number(s) O43253 Q86U01 Q9BV62
Sequence was last modified on March 2, 2010 (version 3)
Annotations were last modified on October 19, 2011 (version 122)
Name and origin of the protein
DescriptionRecName: Full=Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; Short=PEPCK-M; EC=4.1.1.32; AltName: Full=Phosphoenolpyruvate carboxylase; Flags: Precursor;
Gene nameName=PCK2
Synonyms=PEPCK2
Encoded onName=PCK2; Synonyms=PEPCK2
KeywordsAlternative splicing; Complete proteome; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Mitochondrion; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX92720; CAA63380.1; -; mRNA
EMBLY11484; CAA72272.1; -; Genomic_DNA
EMBLBX248272; CAD62600.1; ALT_INIT; mRNA
EMBLAL136295; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBC001454; AAH01454.1; -; mRNA
IPIIPI00384116; -; .
IPIIPI00797038; -; .
PIRS69546; S69546; .
RefSeqNP_001018083.1; NM_001018073.1; .
RefSeqNP_004554.2; NM_004563.2; .
UniGeneHs.75812; -; .
ProteinModelPortalQ16822; -; .
SMRQ16822; 31-640; .
IntActQ16822; 5; .
STRINGQ16822; -; .
PhosphoSiteQ16822; -; .
SWISS-2DPAGEQ16822; -; .
PRIDEQ16822; -; .
EnsemblENST00000216780; ENSP00000216780; ENSG00000100889; .
GeneID5106; -; .
KEGGhsa:5106; -; .
CTD5106; -; .
GeneCardsGC14P004678; -; .
HGNCHGNC:8725; PCK2; .
HPACAB018734; -; .
MIM261650; phenotype; .
MIM614095; gene; .
neXtProtNX_Q16822; -; .
Orphanet2880; Phosphoenolpyruvate carboxykinase deficiency; .
PharmGKBPA33070; -; .
eggNOGprNOG17554; -; .
HOGENOMHBG484437; -; .
HOVERGENHBG053651; -; .
InParanoidQ16822; -; .
OMAQAVDERF; -; .
OrthoDBEOG47SSD8; -; .
ReactomeREACT_474; Metabolism of carbohydrates; .
NextBio19706; -; .
ArrayExpressQ16822; -; .
BgeeQ16822; -; .
CleanExHS_PCK2; -; .
GenevestigatorQ16822; -; .
GermOnlineENSG00000100889; Homo sapiens; .
GOGO:0005759; C:mitochondrial matrix; TAS:Reactome; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:EC; .
GOGO:0006094; P:gluconeogenesis; TAS:Reactome; .
InterProIPR018091; PEP_carboxykin_GTP_CS; .
InterProIPR013035; PEP_carboxykinase_C; .
InterProIPR008209; PEP_carboxykinase_GTP; .
InterProIPR008210; PEP_carboxykinase_N; .
Gene3DG3DSA:3.90.228.20; PEP_carboxykinase_C; 2; .
Gene3DG3DSA:3.40.449.10; PEP_carboxykinase_N; 1; .
PANTHERPTHR11561; PEP_carboxykin; 1; .
PfamPF00821; PEPCK; 1; .
PIRSFPIRSF001348; PEP_carboxykinase_GTP; 1; .
SUPFAMSSF68923; PEP_carboxykinase_N; 1; .
PROSITEPS00505; PEPCK_GTP; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server