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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DHSA_ECOLI




SWISS-2DPAGE:  DHSA_ECOLI


DHSA_ECOLI


General information about the entry
View entry in simple text format
Entry nameDHSA_ECOLI
Primary accession numberP0AC41
Secondary accession number(s) P10444
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Succinate dehydrogenase flavoprotein subunit; EC=1.3.99.1;.
Gene nameName=sdhA
OrderedLocusNames=b0723, JW0713
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
Comments
  • SUBUNIT: HETEROTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.74; Mw=63723
pI=5.82; Mw=64101
pI=5.88; Mw=63346

MAPPING (identification):
SPOTS 2D-000KJL, 2D-000KJP: AMINO ACID COMPOSITION AND SEQUENCE TAG (MKLP) [3];
SPOT 2D-000KJN: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEF060.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0AC41; DHSA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDHSA_ECOLI
Primary accession numberP0AC41
Secondary accession number(s) P10444 P78282
Sequence was last modified on November 8, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 63)
Name and origin of the protein
DescriptionRecName: Full=Succinate dehydrogenase flavoprotein subunit; EC=1.3.99.1;
Gene nameName=sdhA
OrderedLocusNames=b0723, JW0713
Encoded onName=sdhA; OrderedLocusNames=b0723, JW0713
Keywords3D-structure; Acetylation; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Oxidoreductase; Reference proteome; Transport; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01619; AAA23895.1; -; Genomic_DNA
EMBLX00980; CAA25487.1; -; Genomic_DNA
EMBLU00096; AAC73817.1; -; Genomic_DNA
EMBLAP009048; BAA35390.1; -; Genomic_DNA
EMBLX01070; CAA25533.1; -; Genomic_DNA
PIRB64808; DEECSF; .
RefSeqNP_415251.1; NC_000913.2; .
PDB1NEK; X-ray; 2.60 A; A=1-588
PDB1NEN; X-ray; 2.90 A; A=1-588
PDB2ACZ; X-ray; 3.10 A; A=1-588
PDB2AD0; Model; -; A=1-588
PDB2WDQ; X-ray; 2.40 A; A/E/I=1-588
PDB2WDR; X-ray; 3.20 A; A/E/I=1-588
PDB2WDV; X-ray; 3.20 A; A/E/I=1-588
PDB2WP9; X-ray; 2.70 A; A/E/I=1-588
PDB2WS3; X-ray; 3.20 A; A/E/I=1-588
PDB2WU2; X-ray; 2.50 A; A/E/I=1-588
PDB2WU5; X-ray; 2.80 A; A/E/I=1-588
PDBsum1NEK; -; .
PDBsum1NEN; -; .
PDBsum2ACZ; -; .
PDBsum2AD0; -; .
PDBsum2WDQ; -; .
PDBsum2WDR; -; .
PDBsum2WDV; -; .
PDBsum2WP9; -; .
PDBsum2WS3; -; .
PDBsum2WU2; -; .
PDBsum2WU5; -; .
ProteinModelPortalP0AC41; -; .
SMRP0AC41; 1-588; .
IntActP0AC41; 90; .
SWISS-2DPAGEP0AC41; -; .
ECO2DBASEF060.3; 6TH EDITION; .
PRIDEP0AC41; -; .
EnsemblBacteriaEBESCT00000001155; EBESCP00000001155; EBESCG00000000952; .
EnsemblBacteriaEBESCT00000001156; EBESCP00000001156; EBESCG00000000952; .
EnsemblBacteriaEBESCT00000018021; EBESCP00000017312; EBESCG00000017077; .
GeneID945402; -; .
GenomeReviewsAP009048_GR; JW0713; .
GenomeReviewsU00096_GR; b0723; .
KEGGecj:JW0713; -; .
KEGGeco:b0723; -; .
EchoBASEEB0924; -; .
EcoGeneEG10931; sdhA; .
eggNOGCOG1053; -; .
GeneTreeEBGT00050000009824; -; .
HOGENOMHBG293998; -; .
OMAAHSRFDY; -; .
ProtClustDBPRK08958; -; .
BioCycEcoCyc:SDH-FLAVO; -; .
BioCycMetaCyc:SDH-FLAVO; -; .
GenevestigatorP0AC41; -; .
GOGO:0045282; C:plasma membrane succinate dehydrogenase complex; IDA:EcoliWiki; .
GOGO:0009055; F:electron carrier activity; IDA:EcoCyc; .
GOGO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoliWiki; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0000104; F:succinate dehydrogenase activity; IMP:EcoliWiki; .
GOGO:0022900; P:electron transport chain; IEA:UniProtKB-KW; .
GOGO:0006810; P:transport; IEA:UniProtKB-KW; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:EcoliWiki; .
InterProIPR003953; FAD_bind2_N; .
InterProIPR013027; FAD_pyr_nucl-diS_OxRdtase; .
InterProIPR003952; FRD_SDH_FAD_BS; .
InterProIPR015939; Fum_Rdtase/Succ_DH_flav-like_C; .
InterProIPR004112; Fum_Rdtase/Succ_DH_flav_C; .
InterProIPR011281; Succ_DH_flav_su_fwd; .
InterProIPR014006; Succ_Dhase_FrdA_Gneg; .
Gene3DG3DSA:1.20.58.100; Fum_Rdtase/Succ_DH_flav-like_C; 1; .
PANTHERPTHR11632:SF5; PTHR11632:SF5; 1; .
PfamPF00890; FAD_binding_2; 1; .
PfamPF02910; Succ_DH_flav_C; 1; .
PRINTSPR00368; FADPNR; .
SUPFAMSSF46977; Succ_DH_flav_C; 1; .
TIGRFAMsTIGR01816; SdhA_forward; 1; .
TIGRFAMsTIGR01812; SdhA_frdA_Gneg; 1; .
PROSITEPS00504; FRD_SDH_FAD_BINDING; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server