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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6L2




SWISS-2DPAGE:  P0A6L2


P0A6L2


General information about the entry
View entry in simple text format
Entry nameDAPA_ECOLI
Primary accession numberP0A6L2
Secondary accession number(s) P05640
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Dihydrodipicolinate synthase; Short=DHDPS; EC=4.2.1.52;.
Gene nameName=dapA
OrderedLocusNames=b2478, JW2463
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.94; Mw=32970  [identification data]
pI=6.09; Mw=32408

MAPPING (identification):
SPOT 2D-000L8E: AMINO ACID COMPOSITION AND SEQUENCE TAG (MFTG) [3];
SPOT 2D-000L8W: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEG031.3; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6L2; DAPA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDAPA_ECOLI
Primary accession numberP0A6L2
Secondary accession number(s) P05640 P78223
Sequence was last modified on May 10, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 65)
Name and origin of the protein
DescriptionRecName: Full=Dihydrodipicolinate synthase; Short=DHDPS; EC=4.2.1.52;
Gene nameName=dapA
OrderedLocusNames=b2478, JW2463
Encoded onName=dapA; OrderedLocusNames=b2478, JW2463
Keywords3D-structure; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Diaminopimelate biosynthesis; Direct protein sequencing; Lyase; Lysine biosynthesis; Reference proteome; Schiff base.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM12844; AAA23665.1; -; Genomic_DNA
EMBLU00096; AAC75531.1; -; Genomic_DNA
EMBLAP009048; BAA16355.1; -; Genomic_DNA
EMBLM33928; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLX57402; CAA40660.1; -; Genomic_DNA
PIRE65023; SYECDP; .
RefSeqNP_416973.1; NC_000913.2; .
PDB1DHP; X-ray; 2.30 A; A/B=1-292
PDB1S5T; X-ray; 2.30 A; A/B=1-292
PDB1S5V; X-ray; 2.35 A; A/B=1-292
PDB1S5W; X-ray; 2.32 A; A/B=1-292
PDB1YXC; X-ray; 1.90 A; A/B=1-292
PDB1YXD; X-ray; 2.00 A; A/B=1-292
PDB2A6L; X-ray; 2.05 A; A/B=1-292
PDB2A6N; X-ray; 1.94 A; A/B=1-292
PDB2ATS; X-ray; 1.90 A; A/B=1-292
PDB2OJP; X-ray; 1.70 A; A/B=1-292
PDB2PUR; X-ray; 1.70 A; A/B=1-292
PDB3C0J; X-ray; 2.40 A; A/B=1-292
PDB3DEN; X-ray; 2.20 A; A/B=1-292
PDB3DU0; X-ray; 2.00 A; A/B=1-292
PDB3I7Q; X-ray; 2.00 A; A/B=1-292
PDB3I7R; X-ray; 2.10 A; A/B=1-292
PDB3I7S; X-ray; 2.30 A; A/B=1-292
PDBsum1DHP; -; .
PDBsum1S5T; -; .
PDBsum1S5V; -; .
PDBsum1S5W; -; .
PDBsum1YXC; -; .
PDBsum1YXD; -; .
PDBsum2A6L; -; .
PDBsum2A6N; -; .
PDBsum2ATS; -; .
PDBsum2OJP; -; .
PDBsum2PUR; -; .
PDBsum3C0J; -; .
PDBsum3DEN; -; .
PDBsum3DU0; -; .
PDBsum3I7Q; -; .
PDBsum3I7R; -; .
PDBsum3I7S; -; .
ProteinModelPortalP0A6L2; -; .
SMRP0A6L2; 1-292; .
IntActP0A6L2; 3; .
SWISS-2DPAGEP0A6L2; -; .
ECO2DBASEG031.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003863; EBESCP00000003863; EBESCG00000003158; .
EnsemblBacteriaEBESCT00000017618; EBESCP00000016909; EBESCG00000016674; .
GeneID946952; -; .
GenomeReviewsAP009048_GR; JW2463; .
GenomeReviewsU00096_GR; b2478; .
KEGGecj:JW2463; -; .
KEGGeco:b2478; -; .
EchoBASEEB0201; -; .
EcoGeneEG10205; dapA; .
eggNOGCOG0329; -; .
GeneTreeEBGT00050000008975; -; .
HOGENOMHBG358848; -; .
OMAFMLCGGH; -; .
ProtClustDBPRK03170; -; .
BioCycEcoCyc:DIHYDRODIPICSYN-MONOMER; -; .
BioCycMetaCyc:DIHYDRODIPICSYN-MONOMER; -; .
GenevestigatorP0A6L2; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0008840; F:dihydrodipicolinate synthase activity; IDA:EcoliWiki; .
GOGO:0019877; P:diaminopimelate biosynthetic process; IDA:EcoliWiki; .
HAMAPMF_00418; DapA; 1; -
InterProIPR013785; Aldolase_TIM; .
InterProIPR002220; Dihydrodipicolinate_synth-like; .
InterProIPR020625; Dihydrodipicolinate_synth_AS; .
InterProIPR020624; Dihydrodipicolinate_synth_CS; .
InterProIPR005263; Dihydrodipicolinate_synth_DapA; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PANTHERPTHR12128; DHDPS; 1; .
PfamPF00701; DHDPS; 1; .
PIRSFPIRSF001365; DHDPS; 1; .
PRINTSPR00146; DHPICSNTHASE; .
TIGRFAMsTIGR00674; DapA; 1; .
PROSITEPS00665; DHDPS_1; 1; .
PROSITEPS00666; DHDPS_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server