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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PHOE_ECOLI




SWISS-2DPAGE:  PHOE_ECOLI


PHOE_ECOLI


General information about the entry
View entry in simple text format
Entry namePHOE_ECOLI
Primary accession numberP02932
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane pore protein E; Flags: Precursor;.
Gene nameName=phoE
Synonyms=ompE
OrderedLocusNames=b0241, JW0231
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.71; Mw=35814

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB037.0; 6TH EDITION.
UniProtKB/Swiss-ProtP02932; PHOE_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePHOE_ECOLI
Primary accession numberP02932
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 121)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane pore protein E; Flags: Precursor;
Gene nameName=phoE
Synonyms=ompE
OrderedLocusNames=b0241, JW0231
Encoded onName=phoE; Synonyms=ompE; OrderedLocusNames=b0241, JW0231
Keywords3D-structure; Cell membrane; Cell outer membrane; Complete proteome; Ion transport; Membrane; Porin; Reference proteome; Signal; Stress response; Transmembrane; Transmembrane beta strand; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00316; CAA23605.1; -; Genomic_DNA
EMBLU70214; AAB08661.1; -; Genomic_DNA
EMBLU00096; AAC73345.1; -; Genomic_DNA
EMBLAP009048; BAA77910.1; -; Genomic_DNA
EMBLX00786; CAA25362.1; -; Genomic_DNA
PIRA03432; MMECPE; .
RefSeqNP_414776.1; NC_000913.2; .
PDB1PHO; X-ray; 3.00 A; A=22-351
PDBsum1PHO; -; .
ProteinModelPortalP02932; -; .
SMRP02932; 22-351; .
DIPDIP-10498N; -; .
IntActP02932; 3; .
MINTMINT-1285942; -; .
TCDB1.B.1.1.2; general bacterial porin (GBP) family; .
SWISS-2DPAGEP02932; -; .
ECO2DBASEB037.0; 6TH EDITION; .
PRIDEP02932; -; .
EnsemblBacteriaEBESCT00000003809; EBESCP00000003809; EBESCG00000003114; .
EnsemblBacteriaEBESCT00000017595; EBESCP00000016886; EBESCG00000016651; .
GeneID944926; -; .
GenomeReviewsAP009048_GR; JW0231; .
GenomeReviewsU00096_GR; b0241; .
KEGGecj:JW0231; -; .
KEGGeco:b0241; -; .
EchoBASEEB0722; -; .
EcoGeneEG10729; phoE; .
eggNOGCOG3203; -; .
GeneTreeEBGT00050000008697; -; .
HOGENOMHBG673855; -; .
OMAKYIDVGA; -; .
ProtClustDBPRK10159; -; .
BioCycEcoCyc:MONOMER0-282; -; .
GenevestigatorP02932; -; .
GOGO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-KW; .
GOGO:0046930; C:pore complex; IEA:UniProtKB-KW; .
GOGO:0015288; F:porin activity; IEA:UniProtKB-KW; .
GOGO:0006811; P:ion transport; IEA:UniProtKB-KW; .
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
InterProIPR017690; OM_insertion_C_omp85_target; .
InterProIPR023614; Porin_dom; .
InterProIPR001897; Porin_gammaproteobac; .
InterProIPR001702; Porin_Gram-ve; .
InterProIPR013793; Porin_Gram-ve_CS; .
Gene3DG3DSA:2.40.160.10; Porin_Gram-ve; 1; .
PfamPF00267; Porin_1; 1; .
PRINTSPR00183; ECOLIPORIN; .
PRINTSPR00182; ECOLNEIPORIN; .
TIGRFAMsTIGR03304; OMP85_target; 2; .
PROSITEPS00576; GRAM_NEG_PORIN; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server