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SWISS-2DPAGE

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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE U937_HUMAN 
Macrophage Like Cell Line  {Homo sapiens (Human)}
tissue: Histiocytic lymphoma


(map ID: 23cfe9a8-3dce-4a90-9b7e-da2abba4727d)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for u937_human   42 identified spots / 32 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods References
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-000CXH
5.22
41'605
{Gm}
1
ATP5D RecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor; P30049
(ATPD_HUMAN)
2D-000D63
2D-000D64
4.22
4.25
12'868
12'755
{Gm}
{Gm}
1
1
BLVRB RecName: Full=Flavin reductase (NADPH); Short=FR; EC=1.5.1.30; AltName: Full=Biliverdin reductase B; Short=BVR-B; EC=1.3.1.24; AltName: Full=Biliverdin-IX beta-reductase; AltName: Full=Green heme-binding protein; Short=GHBP; AltName: Full=NADPH-dependent diaphorase; AltName: Full=NADPH-flavin reductase; Short=FLR; P30043
(BLVRB_HUMAN)
2D-000D2W
2D-000D56
6.33
7.34
29'717
22'307
{Gm}
{Gm}
1
1
C21orf33 RecName: Full=ES1 protein homolog, mitochondrial; AltName: Full=Protein GT335; AltName: Full=Protein KNP-I; Flags: Precursor; P30042
(ES1_HUMAN)
2D-000D4L
6.90
24'306
{Gm}
1
CALR RecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor; P27797
(CALR_HUMAN)
2D-000CRG
3.73
58'084
{Gm}
1
CMPK1 RecName: Full=UMP-CMP kinase; EC=2.7.4.14; AltName: Full=Cytidine monophosphate kinase; AltName: Full=Cytidylate kinase; AltName: Full=Deoxycytidylate kinase; AltName: Full=Uridine monophosphate kinase; AltName: Full=Uridine monophosphate/cytidine monophosphate kinase; Short=UMP/CMP kinase; Short=UMP/CMPK; P30085
(KCY_HUMAN)
2D-000D59
5.41
21'450
{Gm}
1
CTSD RecName: Full=Cathepsin D; EC=3.4.23.5; Contains: RecName: Full=Cathepsin D light chain; Contains: RecName: Full=Cathepsin D heavy chain; Flags: Precursor; P07339
(CATD_HUMAN)
2D-000D2Y
5.50
29'497
{Gm}
1
ECHS1 RecName: Full=Enoyl-CoA hydratase, mitochondrial; EC=4.2.1.17; AltName: Full=Enoyl-CoA hydratase 1; AltName: Full=Short-chain enoyl-CoA hydratase; Short=SCEH; Flags: Precursor; P30084
(ECHM_HUMAN)
2D-000D40
5.86
26'133
{Gm}
1
EEF1B2 RecName: Full=Elongation factor 1-beta; Short=EF-1-beta; P24534
(EF1B_HUMAN)
2D-000D2X
4.47
29'497
{Gm}
1
ERP29 RecName: Full=Endoplasmic reticulum resident protein 29; Short=ERp29; AltName: Full=Endoplasmic reticulum resident protein 28; Short=ERp28; AltName: Full=Endoplasmic reticulum resident protein 31; Short=ERp31; Flags: Precursor; P30040
(ERP29_HUMAN)
2D-000D3S
5.55
26'787
{Gm}
1
GAPDH RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-; P04406
(G3P_HUMAN)
2D-000D19
2D-000D14
2D-000D18
2D-000D11
8.24
8.69
7.91
8.95
35'243
35'414
35'300
35'643
{Gm}
{Gm}
{Gm}
{Gm}
1
1
1
1
GLUD1 RecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor; P00367
(DHE3_HUMAN)
2D-000CV1
6.84
48'637
{Gm}
1
GSTP1 RecName: Full=Glutathione S-transferase P; EC=2.5.1.18; AltName: Full=GST class-pi; AltName: Full=GSTP1-1; P09211
(GSTP1_HUMAN)
2D-000D4X
5.44
23'328
{Gm}
1
HIBADH RecName: Full=3-hydroxyisobutyrate dehydrogenase, mitochondrial; Short=HIBADH; EC=1.1.1.31; Flags: Precursor; P31937
(3HIDH_HUMAN)
2D-000D2Z
5.51
29'570
{Gm}
1
P4HB RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor; P07237
(PDIA1_HUMAN)
2D-000CSH
4.81
54'949
{Gm}
1
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-000CTP
5.54
51'950
{Gm}
1
PEBP1 RecName: Full=Phosphatidylethanolamine-binding protein 1; Short=PEBP-1; AltName: Full=HCNPpp; AltName: Full=Neuropolypeptide h3; AltName: Full=Prostatic-binding protein; AltName: Full=Raf kinase inhibitor protein; Short=RKIP; Contains: RecName: Full=Hippocampal cholinergic neurostimulating peptide; Short=HCNP; P30086
(PEBP1_HUMAN)
2D-000D5H
7.36
19'612
{Gm}
1
PPIA RecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A; EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin A-binding protein; AltName: Full=Rotamase A; P62937
(PPIA_HUMAN)
2D-000D5Z
2D-000D5X
7.44
7.15
13'808
13'972
{Gm}
{Gm}
1
1
PRDX3 RecName: Full=Thioredoxin-dependent peroxide reductase, mitochondrial; EC=1.11.1.15; AltName: Full=Antioxidant protein 1; Short=AOP-1; AltName: Full=HBC189; AltName: Full=Peroxiredoxin III; Short=Prx-III; AltName: Full=Peroxiredoxin-3; AltName: Full=Protein MER5 homolog; Flags: Precursor; P30048
(PRDX3_HUMAN)
2D-000D4U
6.02
23'591
{Gm}
1
PRDX5 RecName: Full=Peroxiredoxin-5, mitochondrial; EC=1.11.1.15; AltName: Full=Alu corepressor 1; AltName: Full=Antioxidant enzyme B166; Short=AOEB166; AltName: Full=Liver tissue 2D-page spot 71B; AltName: Full=PLP; AltName: Full=Peroxiredoxin V; Short=Prx-V; AltName: Full=Peroxisomal antioxidant enzyme; AltName: Full=TPx type VI; AltName: Full=Thioredoxin peroxidase PMP20; AltName: Full=Thioredoxin reductase; Flags: Precursor; P30044
(PRDX5_HUMAN)
2D-000D5Y
6.95
13'972
{Gm}
1
PRDX6 RecName: Full=Peroxiredoxin-6; EC=1.11.1.15; AltName: Full=1-Cys peroxiredoxin; Short=1-Cys PRX; AltName: Full=24 kDa protein; AltName: Full=Acidic calcium-independent phospholipase A2; Short=aiPLA2; EC=3.1.1.-; AltName: Full=Antioxidant protein 2; AltName: Full=Liver 2D page spot 40; AltName: Full=Non-selenium glutathione peroxidase; Short=NSGPx; EC=1.11.1.9; AltName: Full=Red blood cells page spot 12; P30041
(PRDX6_HUMAN)
2D-000D45
6.27
25'748
{Gm}
1
PSMB4 RecName: Full=Proteasome subunit beta type-4; Short=Proteasome beta chain; EC=3.4.25.1; AltName: Full=26 kDa prosomal protein; Short=HsBPROS26; Short=PROS-26; AltName: Full=Macropain beta chain; AltName: Full=Multicatalytic endopeptidase complex beta chain; AltName: Full=Proteasome chain 3; Short=HsN3; Flags: Precursor; P28070
(PSB4_HUMAN)
2D-000D4M
5.50
24'255
{Gm}
1
PSMB6 RecName: Full=Proteasome subunit beta type-6; EC=3.4.25.1; AltName: Full=Macropain delta chain; AltName: Full=Multicatalytic endopeptidase complex delta chain; AltName: Full=Proteasome delta chain; AltName: Full=Proteasome subunit Y; Flags: Precursor; P28072
(PSB6_HUMAN)
2D-000D4Z
4.90
22'748
{Gm}
1
SOD1 RecName: Full=Superoxide dismutase [Cu-Zn]; EC=1.15.1.1; AltName: Full=Superoxide dismutase 1; Short=hSod1; P00441
(SODC_HUMAN)
2D-000D5L
5.56
15'854
{Gm}
1
SOD2 RecName: Full=Superoxide dismutase [Mn], mitochondrial; EC=1.15.1.1; Flags: Precursor; P04179
(SODM_HUMAN)
2D-000D57
6.95
21'935
{Gm}
1
SPTB RecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin; P11277
(SPTB1_HUMAN)
2D-000D47
5.52
25'584
{Gm}
1
TF RecName: Full=Serotransferrin; Short=Transferrin; AltName: Full=Beta-1 metal-binding globulin; AltName: Full=Siderophilin; Flags: Precursor; P02787
(TRFE_HUMAN)
2D-000CM8
2D-000CM4
6.93
6.86
99'201
99'201
{Gm}
{Gm}
1
1
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P60174
(TPIS_HUMAN)
2D-000D48
2D-000D4A
2D-000D46
6.81
6.64
6.51
25'475
25'420
25'638
{Gm}
{Gm}
{Gm}
1
1
1
TPT1 RecName: Full=Translationally-controlled tumor protein; Short=TCTP; AltName: Full=Fortilin; AltName: Full=Histamine-releasing factor; Short=HRF; AltName: Full=p23; P13693
(TCTP_HUMAN)
2D-000D58
4.87
21'874
{Gm}
1
TUBB2C RecName: Full=Tubulin beta-2C chain; AltName: Full=Tubulin beta-2 chain; P68371
(TBB2C_HUMAN)
2D-000D26
5.33
31'689
{Gm}
1
TXN RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor; Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP; P10599
(THIO_HUMAN)
2D-000D6S
4.93
10'599
{Gm}
1
UBC RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor; P0CG48
(UBC_HUMAN)
2D-000D77
2D-000D76
6.90
6.85
8'844
8'881
{Gm}
{Gm}
1
1
Mapping legends: { Gm } => 'Gel matching'



Cited references

# PubMed/Medline Authors Location
[1] 7498157 Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.; Electrophoresis 16(1):1131-1151(1995).
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Gel experimental information

Information Type Data
Map name U937_HUMAN {Macrophage Like Cell Line}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] U937 [Homo sapiens (Human) / TaxID:9606]
Tissue (Swiss-Prot definition) Histiocytic lymphoma
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 895
Number of identified spots 42
Number of identified proteins 32
Reference Bibliographic reference
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server