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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00359




SWISS-2DPAGE:  P00359


P00359


General information about the entry
View entry in simple text format
Entry nameG3P3_YEAST
Primary accession numberP00359
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 2)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 3; Short=GAPDH 3; EC=1.2.1.12;.
Gene nameName=TDH3
Synonyms=GPD3
OrderedLocusNames=YGR192C
ORFNames=G7576
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=86059345; PubMed=2999100; [NCBI, ExPASy, EBI, Israel, Japan]
McAlister L., Holland M.J.
''''''Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes'';'';''
J. Biol. Chem. 260(1):15019-15027(1985)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.81; Mw=32772
pI=6.73; Mw=32772
pI=6.88; Mw=32607

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY MCALLISTER AND HOLLAND [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP00359; G3P3_YEAST.
UniProtKB/Swiss-ProtP00359; G3P3_YEAST.
YEPD4280.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameG3P3_YEAST
Primary accession numberP00359
Secondary accession number(s) D6VUX4 Q6Q5P9
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 133)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 3; Short=GAPDH 3; EC=1.2.1.12;
Gene nameName=TDH3
Synonyms=GPD3
OrderedLocusNames=YGR192C
ORFNames=G7576
Encoded onName=TDH3; Synonyms=GPD3; OrderedLocusNames=YGR192C; ORFNames=G7576
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV01300; CAA24607.1; -; Genomic_DNA
EMBLJ01324; AAA88714.1; -; Genomic_DNA
EMBLX82408; CAA57803.1; -; Genomic_DNA
EMBLZ72977; CAA97218.1; -; Genomic_DNA
EMBLAY557831; AAS56157.1; -; Genomic_DNA
EMBLBK006941; DAA08285.1; -; Genomic_DNA
PIRS55870; DEBYG2; .
RefSeqNP_011708.1; NM_001181321.1; .
ProteinModelPortalP00359; -; .
SMRP00359; 1-330; .
DIPDIP-4309N; -; .
IntActP00359; 93; .
MINTMINT-567189; -; .
STRINGP00359; -; .
SWISS-2DPAGEP00359; -; .
COMPLUYEAST-2DPAGEP00359; -; .
PeptideAtlasP00359; -; .
EnsemblFungiYGR192C; YGR192C; YGR192C; .
GeneID853106; -; .
KEGGsce:YGR192C; -; .
NMPDRfig|4932.3.peg.2835; -; .
SGDS000003424; TDH3; .
eggNOGfuNOG04196; -; .
GeneTreeEFGT00050000004847; -; .
HOGENOMHBG571736; -; .
OMASAYAPMF; -; .
OrthoDBEOG4578GC; -; .
PhylomeDBP00359; -; .
NextBio973112; -; .
ArrayExpressP00359; -; .
GenevestigatorP00359; -; .
GermOnlineYGR192C; Saccharomyces cerevisiae; .
GOGO:0009277; C:fungal-type cell wall; IDA:SGD; .
GOGO:0005811; C:lipid particle; IDA:SGD; .
GOGO:0005739; C:mitochondrion; IDA:SGD; .
GOGO:0001950; C:plasma membrane enriched fraction; IDA:SGD; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:SGD; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006915; P:apoptosis; IMP:SGD; .
GOGO:0006094; P:gluconeogenesis; IEP:SGD; .
GOGO:0006096; P:glycolysis; IEP:SGD; .
GOGO:0072593; P:reactive oxygen species metabolic process; IMP:SGD; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server