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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00861




SWISS-2DPAGE:  P00861


P00861


General information about the entry
View entry in simple text format
Entry nameDCDA_ECOLI
Primary accession numberP00861
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on November 9, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Diaminopimelate decarboxylase; Short=DAP decarboxylase; Short=DAPDC; EC=4.1.1.20;.
Gene nameName=lysA
OrderedLocusNames=b2838, JW2806
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.76; Mw=45059

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEG043.9; 6TH EDITION.
UniProtKB/Swiss-ProtP00861; DCDA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDCDA_ECOLI
Primary accession numberP00861
Secondary accession number(s) Q2M9Z9
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 109)
Name and origin of the protein
DescriptionRecName: Full=Diaminopimelate decarboxylase; Short=DAP decarboxylase; Short=DAPDC; EC=4.1.1.20;
Gene nameName=lysA
OrderedLocusNames=b2838, JW2806
Encoded onName=lysA; OrderedLocusNames=b2838, JW2806
Keywords3D-structure; Amino-acid biosynthesis; Complete proteome; Decarboxylase; Lyase; Lysine biosynthesis; Pyridoxal phosphate; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01614; AAA83861.1; -; Genomic_DNA
EMBLU29581; AAB40485.1; -; Genomic_DNA
EMBLU00096; AAC75877.1; -; Genomic_DNA
EMBLAP009048; BAE76907.1; -; Genomic_DNA
PIRA01078; DCECD; .
RefSeqNP_417315.1; NC_000913.2; .
PDB1KNW; X-ray; 2.10 A; A=1-420
PDB1KO0; X-ray; 2.20 A; A=1-420
PDBsum1KNW; -; .
PDBsum1KO0; -; .
ProteinModelPortalP00861; -; .
SMRP00861; 2-411; .
DIPDIP-10132N; -; .
IntActP00861; 7; .
MINTMINT-1292495; -; .
SWISS-2DPAGEP00861; -; .
ECO2DBASEG043.9; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004244; EBESCP00000004244; EBESCG00000003462; .
EnsemblBacteriaEBESCT00000016688; EBESCP00000015979; EBESCG00000015747; .
GeneID947313; -; .
GenomeReviewsAP009048_GR; JW2806; .
GenomeReviewsU00096_GR; b2838; .
KEGGecj:JW2806; -; .
KEGGeco:b2838; -; .
EchoBASEEB0544; -; .
EcoGeneEG10549; lysA; .
eggNOGCOG0019; -; .
GeneTreeEBGT00050000010955; -; .
HOGENOMHBG672375; -; .
OMAAIDTEHY; -; .
ProtClustDBPRK11165; -; .
BioCycEcoCyc:DIAMINOPIMDECARB-MONOMER; -; .
BioCycMetaCyc:DIAMINOPIMDECARB-MONOMER; -; .
GenevestigatorP00861; -; .
GOGO:0008836; F:diaminopimelate decarboxylase activity; IEA:EC; .
GOGO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:InterPro; .
HAMAPMF_02120; LysA; 1; -
InterProIPR009006; Ala_racemase/Decarboxylase_C; .
InterProIPR002986; DAP_deCOOHase_LysA; .
InterProIPR022643; De-COase2_C; .
InterProIPR022657; De-COase2_CS; .
InterProIPR022644; De-COase2_N; .
InterProIPR022653; De-COase2_pyr-phos_BS; .
InterProIPR000183; Orn/DAP/Arg_de-COase; .
Gene3DG3DSA:2.40.37.10; Ala_racemase/Decarboxylase_C; 1; .
PfamPF02784; Orn_Arg_deC_N; 1; .
PfamPF00278; Orn_DAP_Arg_deC; 1; .
PRINTSPR01181; DAPDCRBXLASE; .
PRINTSPR01179; ODADCRBXLASE; .
SUPFAMSSF50621; Racem_decarbox_C; 1; .
TIGRFAMsTIGR01048; LysA; 1; .
PROSITEPS00878; ODR_DC_2_1; 1; .
PROSITEPS00879; ODR_DC_2_2; 1; .



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