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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00957




SWISS-2DPAGE:  P00957


P00957


General information about the entry
View entry in simple text format
Entry nameSYA_ECOLI
Primary accession numberP00957
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Alanyl-tRNA synthetase; EC=6.1.1.7; AltName: Full=Alanine--tRNA ligase; Short=AlaRS;.
Gene nameName=alaS
Synonyms=lovB
OrderedLocusNames=b2697, JW2667
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.50; Mw=96123

MAPPING (identification):
GEL MATCHING [1] AND TAGGING AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEF093.0; 6TH EDITION.
UniProtKB/Swiss-ProtP00957; SYA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameSYA_ECOLI
Primary accession numberP00957
Secondary accession number(s) P78279
Sequence was last modified on November 1, 1997 (version 2)
Annotations were last modified on October 19, 2011 (version 121)
Name and origin of the protein
DescriptionRecName: Full=Alanyl-tRNA synthetase; EC=6.1.1.7; AltName: Full=Alanine--tRNA ligase; Short=AlaRS;
Gene nameName=alaS
Synonyms=lovB
OrderedLocusNames=b2697, JW2667
Encoded onName=alaS; Synonyms=lovB; OrderedLocusNames=b2697, JW2667
Keywords3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding; tRNA-binding; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU00096; AAC75739.1; -; Genomic_DNA
EMBLAP009048; BAA16559.1; -; Genomic_DNA
EMBLJ01581; AAA03208.1; -; Unassigned_DNA
EMBLL07596; AAA71918.1; ALT_SEQ; Unassigned_DNA
EMBLD44453; BAA21554.1; -; Genomic_DNA
EMBLZ28405; CAA82247.1; -; Genomic_DNA
PIRE65049; SYECAT; .
RefSeqNP_417177.1; NC_000913.2; .
PDB3HXU; X-ray; 2.10 A; A=2-442
PDB3HXV; X-ray; 1.93 A; A=2-442
PDB3HXW; X-ray; 1.93 A; A=2-442
PDB3HXX; X-ray; 2.11 A; A=2-442
PDB3HXY; X-ray; 2.27 A; A=2-442
PDB3HXZ; X-ray; 1.99 A; A/B/C/D=2-442
PDB3HY0; X-ray; 1.90 A; A/B=2-442
PDB3HY1; X-ray; 2.79 A; A/B=2-442
PDBsum3HXU; -; .
PDBsum3HXV; -; .
PDBsum3HXW; -; .
PDBsum3HXX; -; .
PDBsum3HXY; -; .
PDBsum3HXZ; -; .
PDBsum3HY0; -; .
PDBsum3HY1; -; .
ProteinModelPortalP00957; -; .
SMRP00957; 2-875; .
DIPDIP-9080N; -; .
IntActP00957; 19; .
MINTMINT-1266342; -; .
SWISS-2DPAGEP00957; -; .
ECO2DBASEF093.0; 6TH EDITION; .
PRIDEP00957; -; .
EnsemblBacteriaEBESCT00000002492; EBESCP00000002492; EBESCG00000002032; .
EnsemblBacteriaEBESCT00000014987; EBESCP00000014278; EBESCG00000014047; .
GeneID947175; -; .
GenomeReviewsAP009048_GR; JW2667; .
GenomeReviewsU00096_GR; b2697; .
KEGGecj:JW2667; -; .
KEGGeco:b2697; -; .
EchoBASEEB0033; -; .
EcoGeneEG10034; alaS; .
eggNOGCOG0013; -; .
GeneTreeEBGT00050000010345; -; .
HOGENOMHBG354397; -; .
OMAEIDIFRT; -; .
ProtClustDBPRK00252; -; .
BioCycEcoCyc:ALAS-MONOMER; -; .
BioCycMetaCyc:ALAS-MONOMER; -; .
GenevestigatorP00957; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0004813; F:alanine-tRNA ligase activity; IDA:EcoCyc; .
GOGO:0002161; F:aminoacyl-tRNA editing activity; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000049; F:tRNA binding; IEA:UniProtKB-KW; .
GOGO:0008270; F:zinc ion binding; IDA:EcoCyc; .
GOGO:0006419; P:alanyl-tRNA aminoacylation; IDA:EcoCyc; .
GOGO:0045892; P:negative regulation of transcription; DNA-dependent; IDA:EcoCyc
HAMAPMF_00036_B; Ala_tRNA_synth_B; 1; -
InterProIPR002318; Ala-tRNA-synth_IIc; .
InterProIPR018162; Ala-tRNA-synth_IIc_anticod-bd; .
InterProIPR018165; Ala-tRNA-synth_IIc_core; .
InterProIPR018164; Ala-tRNA-synth_IIc_N; .
InterProIPR023033; Ala_tRNA_synth_euk/bac; .
InterProIPR003156; Pesterase_DHHA1; .
InterProIPR018163; Thr/Ala-tRNA-synth_IIc_edit; .
InterProIPR012947; tRNA_SAD; .
PfamPF02272; DHHA1; 1; .
PfamPF01411; tRNA-synt_2c; 1; .
PfamPF07973; tRNA_SAD; 1; .
PRINTSPR00980; TRNASYNTHALA; .
SMARTSM00863; tRNA_SAD; 1; .
SUPFAMSSF101353; Ala-tRNA-synth_IIc_anticod-bd; 1; .
SUPFAMSSF55186; Thr/Ala-tRNA-synth_IIc_edit; 1; .
TIGRFAMsTIGR00344; AlaS; 1; .
PROSITEPS50860; AA_TRNA_LIGASE_II_ALA; 1; .



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