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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P14540




SWISS-2DPAGE:  P14540


P14540


General information about the entry
View entry in simple text format
Entry nameALF_YEAST
Primary accession numberP14540
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Fructose-bisphosphate aldolase; Short=FBP aldolase; Short=FBPA; EC=4.1.2.13; AltName: Full=Fructose-1,6-bisphosphate aldolase;.
Gene nameName=FBA1
OrderedLocusNames=YKL060C
ORFNames=YKL320
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=5.87; Mw=36899
pI=5.99; Mw=37212
pI=5.75; Mw=36899

MAPPING (identification):
AMINO ACID COMPOSITION ANALYSIS, MICROSEQUENCING [1] AND IDENTIFIED ON 2D GELS BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP14540; ALF_YEAST.
UCD-2DPAGEP14540; ALF_YEAST.
UniProtKB/Swiss-ProtP14540; ALF_YEAST.
YEPD6350.
YEPD8332.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameALF_YEAST
Primary accession numberP14540
Secondary accession number(s) D6VXM7
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 119)
Name and origin of the protein
DescriptionRecName: Full=Fructose-bisphosphate aldolase; Short=FBP aldolase; Short=FBPA; EC=4.1.2.13; AltName: Full=Fructose-1,6-bisphosphate aldolase;
Gene nameName=FBA1
OrderedLocusNames=YKL060C
ORFNames=YKL320
Encoded onName=FBA1; OrderedLocusNames=YKL060C; ORFNames=YKL320
KeywordsComplete proteome; Direct protein sequencing; Glycolysis; Lyase; Metal-binding; Phosphoprotein; Reference proteome; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX15003; CAA33111.1; -; Genomic_DNA
EMBLZ28060; CAA81897.1; -; Genomic_DNA
EMBLX75781; CAA53412.1; -; Genomic_DNA
EMBLM32026; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBK006944; DAA09097.1; -; Genomic_DNA
PIRS07855; ADBY2; .
RefSeqNP_012863.1; NM_001179626.1; .
ProteinModelPortalP14540; -; .
SMRP14540; 2-359; .
DIPDIP-4702N; -; .
IntActP14540; 21; .
MINTMINT-564440; -; .
STRINGP14540; -; .
SWISS-2DPAGEP14540; -; .
COMPLUYEAST-2DPAGEP14540; -; .
UCD-2DPAGEP14540; -; .
PeptideAtlasP14540; -; .
EnsemblFungiYKL060C; YKL060C; YKL060C; .
GeneID853805; -; .
KEGGsce:YKL060C; -; .
NMPDRfig|4932.3.peg.3848; -; .
SGDS000001543; FBA1; .
eggNOGfuNOG05260; -; .
GeneTreeEFGT00050000005775; -; .
HOGENOMHBG327581; -; .
OMASPNFSIA; -; .
OrthoDBEOG4J14HR; -; .
PhylomeDBP14540; -; .
NextBio974960; -; .
ArrayExpressP14540; -; .
GenevestigatorP14540; -; .
GermOnlineYKL060C; Saccharomyces cerevisiae; .
GOGO:0005829; C:cytosol; IDA:SGD; .
GOGO:0005739; C:mitochondrion; IDA:SGD; .
GOGO:0004332; F:fructose-bisphosphate aldolase activity; IDA:SGD; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
GOGO:0006094; P:gluconeogenesis; IMP:SGD; .
GOGO:0006096; P:glycolysis; IMP:SGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR006411; Fruct_bisP_bact; .
InterProIPR000771; Ketose_bisP_aldolase_II; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PfamPF01116; F_bP_aldolase; 1; .
PIRSFPIRSF001359; F_bP_aldolase_II; 1; .
TIGRFAMsTIGR00167; CbbA; 1; .
TIGRFAMsTIGR01520; FruBisAldo_II_A; 1; .
PROSITEPS00602; ALDOLASE_CLASS_II_1; 1; .
PROSITEPS00806; ALDOLASE_CLASS_II_2; 1; .



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