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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P32288




SWISS-2DPAGE:  P32288


P32288


General information about the entry
View entry in simple text format
Entry nameGLNA_YEAST
Primary accession numberP32288
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onDecember 30, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase; Short=GS; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase;.
Gene nameName=GLN1
OrderedLocusNames=YPR035W
ORFNames=YP3085.01, YP9367.15
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=83082847; PubMed=6129248; [NCBI, ExPASy, EBI, Israel, Japan]
Mitchell A., Magasanik B.
''''''Purification and properties of glutamine synthetase from Saccharomyces cerevisiae'';'';''
J. Biol. Chem. 258(1):610-615(1983)
Comments
  • SUBUNIT: HOMOOCTAMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.08; Mw=41709

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2D GELS BY MITCHELL AND MAGASANIK [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP32288; GLNA_YEAST.
YEPD5410.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGLNA_YEAST
Primary accession numberP32288
Secondary accession number(s) D6W445 Q03959
Sequence was last modified on September 21, 2011 (version 4)
Annotations were last modified on October 19, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Glutamine synthetase; Short=GS; EC=6.3.1.2; AltName: Full=Glutamate--ammonia ligase;
Gene nameName=GLN1
OrderedLocusNames=YPR035W
ORFNames=YP3085.01, YP9367.15
Encoded onName=GLN1; OrderedLocusNames=YPR035W; ORFNames=YP3085.01, YP9367.15
Keywords3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM65157; AAA34644.1; ALT_SEQ; Genomic_DNA
EMBLZ68111; CAA92141.1; -; Genomic_DNA
EMBLZ71255; CAA94985.1; -; Genomic_DNA
EMBLZ49274; CAA89289.1; -; Genomic_DNA
EMBLBK006949; DAA11461.2; -; Genomic_DNA
PIRS61058; S61058; .
RefSeqNP_015360.2; NM_001184132.2; .
PDB3FKY; X-ray; 2.95 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T=1-370
PDBsum3FKY; -; .
ProteinModelPortalP32288; -; .
SMRP32288; 21-366; .
DIPDIP-6699N; -; .
IntActP32288; 76; .
MINTMINT-658171; -; .
STRINGP32288; -; .
SWISS-2DPAGEP32288; -; .
PeptideAtlasP32288; -; .
EnsemblFungiYPR035W; YPR035W; YPR035W; .
GeneID856147; -; .
KEGGsce:YPR035W; -; .
SGDS000006239; GLN1; .
eggNOGfuNOG05055; -; .
GeneTreeEFGT00050000004376; -; .
HOGENOMHBG299709; -; .
OMAGIMCETV; -; .
OrthoDBEOG4DBXP5; -; .
PhylomeDBP32288; -; .
BioCycMetaCyc:MONOMER-12439; -; .
ArrayExpressP32288; -; .
GenevestigatorP32288; -; .
GermOnlineYPR035W; Saccharomyces cerevisiae; .
GOGO:0005737; C:cytoplasm; IDA:SGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004356; F:glutamate-ammonia ligase activity; IMP:SGD; .
GOGO:0006542; P:glutamine biosynthetic process; IEA:InterPro; .
InterProIPR008147; Gln_synt_beta; .
InterProIPR014746; Gln_synth/guanido_kin_cat_dom; .
InterProIPR008146; Gln_synth_cat_dom; .
Gene3DG3DSA:3.30.590.10; ATP-gua_Ptrans; 1; .
PfamPF00120; Gln-synt_C; 1; .
PfamPF03951; Gln-synt_N; 1; .
SUPFAMSSF54368; Gln_synt_beta; 1; .
PROSITEPS00180; GLNA_1; 1; .
PROSITEPS00181; GLNA_ATP; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server