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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P32471




SWISS-2DPAGE:  P32471


P32471


General information about the entry
View entry in simple text format
Entry nameEF1B_YEAST
Primary accession numberP32471
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 1-beta; Short=EF-1-beta; AltName: Full=Eukaryotic elongation factor 1Balpha; Short=eEF1Balpha; AltName: Full=Translation elongation factor 1B alpha;.
Gene nameName=EFB1
Synonyms=TEF5
OrderedLocusNames=YAL003W
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=95203288; PubMed=7895733; [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
''''''Protein identifications for a Saccharomyces cerevisiae protein database'';'';''
Electrophoresis 15(1):1466-1486(1994)
Comments
  • SUBUNIT: EF-1 IS COMPOSED OF FOUR SUBUNITS: ALPHA, BETA, DELTA, AND GAMMA
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=4.34; Mw=29769

MAPPING (identification):
AMINO ACID COMPOSITION ANALYSIS [1] AND IDENTIFIED BY GARRELS [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP32471; EF1B_YEAST.
YEPD9404.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameEF1B_YEAST
Primary accession numberP32471
Secondary accession number(s) D6VPL3
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on October 19, 2011 (version 113)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 1-beta; Short=EF-1-beta; AltName: Full=Eukaryotic elongation factor 1Balpha; Short=eEF1Balpha; AltName: Full=Translation elongation factor 1B alpha;
Gene nameName=EFB1
Synonyms=TEF5
OrderedLocusNames=YAL003W
Encoded onName=EFB1; Synonyms=TEF5; OrderedLocusNames=YAL003W
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Elongation factor; Phosphoprotein; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD14080; BAA03165.1; -; Genomic_DNA
EMBLL22015; AAC04954.1; -; Genomic_DNA
EMBLBK006935; DAA06983.1; -; Genomic_DNA
PIRS43445; S43445; .
RefSeqNP_009398.1; NM_001178150.1; .
PDB1F60; X-ray; 1.67 A; B=113-206
PDB1G7C; X-ray; 2.05 A; B=113-206
PDB1IJE; X-ray; 2.40 A; B=117-206
PDB1IJF; X-ray; 3.00 A; B=117-206
PDB2B7B; X-ray; 2.60 A; B=113-204
PDB2B7C; X-ray; 1.80 A; B=113-204
PDBsum1F60; -; .
PDBsum1G7C; -; .
PDBsum1IJE; -; .
PDBsum1IJF; -; .
PDBsum2B7B; -; .
PDBsum2B7C; -; .
ProteinModelPortalP32471; -; .
SMRP32471; 10-67; 117-206; .
DIPDIP-6445N; -; .
IntActP32471; 4; .
MINTMINT-441798; -; .
STRINGP32471; -; .
SWISS-2DPAGEP32471; -; .
PeptideAtlasP32471; -; .
EnsemblFungiYAL003W; YAL003W; YAL003W; .
GeneID851260; -; .
KEGGsce:YAL003W; -; .
NMPDRfig|4932.3.peg.82; -; .
SGDS000000003; EFB1; .
eggNOGfuNOG08576; -; .
GeneTreeEFGT00050000005969; -; .
HOGENOMHBG714747; -; .
OMAFSRWFNH; -; .
OrthoDBEOG441TMN; -; .
PhylomeDBP32471; -; .
NextBio968216; -; .
ArrayExpressP32471; -; .
GenevestigatorP32471; -; .
GermOnlineYAL003W; Saccharomyces cerevisiae; .
GOGO:0005853; C:eukaryotic translation elongation factor 1 complex; IMP:SGD; .
GOGO:0005840; C:ribosome; TAS:SGD; .
GOGO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD; .
GOGO:0003746; F:translation elongation factor activity; TAS:SGD; .
InterProIPR018940; EF-1_beta_acid_region_euk; .
InterProIPR010987; Glutathione-S-Trfase_C-like; .
InterProIPR014717; Transl_elong_EF1B/ribosomal_S6; .
InterProIPR001326; Transl_elong_EF1B_B/D_CS; .
InterProIPR014038; Transl_elong_fac_EF1B_bsu/dsu; .
Gene3DG3DSA:1.20.1050.10; GST_C_like; 1; .
Gene3DG3DSA:3.30.70.60; Transl_elong_EF1B/rib_con; 1; .
PfamPF10587; EF-1_beta_acid; 1; .
PfamPF00736; EF1_GNE; 1; .
SMARTSM00888; EF1_GNE; 1; .
SUPFAMSSF54984; Transl_elong_EF1B_B/D_G_exch; 1; .
PROSITEPS00824; EF1BD_1; 1; .
PROSITEPS00825; EF1BD_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server