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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P60010 SWISS-2DPAGE:  P60010 P60010 General information about the entry View entry in simple text format Entry name ACT_YEAST Primary accession number P60010 Secondary accession number(s) P02579 integrated into SWISS-2DPAGE on  February 1, 1995 (release 1) 2D Annotations were last modified on March 31, 2004 (version 1) General Annotations were last modified on  May 19, 2011 (version 12) Name and origin of the protein Description RecName: Full=Actin;. Gene name Name=ACT1 Synonyms=ABY1, END7 OrderedLocusNames=YFL039C Annotated species Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. References [1]   MAPPING ON GEL MEDLINE=96314060; PubMed=8740180; [NCBI, ExPASy, EBI, Israel, Japan] Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F. ''''''The yeast SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):556-565(1996) [2]   MAPPING ON GEL MEDLINE=82271855; PubMed=7050667; [NCBI, ExPASy, EBI, Israel, Japan] Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S. ''''''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'';'';'' Mol. Cell. Biol. 2(1):117-126(1982) [3]   MAPPING ON GEL MEDLINE=89073918; PubMed=3332961; [NCBI, ExPASy, EBI, Israel, Japan] Bataille N., Peypouquet M.-F., Boucherie H. ''''''Identification of polypeptides of the carbon metabolism machinery on the two-dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides'';'';'' Yeast 3(1):11-21(1987) Comments SUBUNIT: MONOMER 2D PAGE maps for identified proteins How to interpret a protein YEAST {Saccharomyces cerevisiae} Saccharomyces cerevisiae (Baker's yeast)   map experimental info   protein estimated location   YEAST MAP LOCATIONS: SPOT 2D-000J9F: pI=5.40; Mw=42600 MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY LUDWIG ET AL [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references UniProtKB/Swiss-Prot P60010; ACT_YEAST. YEPD 7395. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name ACT_YEAST Primary accession number P60010 Secondary accession number(s) D6VTJ1 P02579 Q9P3X6 Q9P3X7 Sequence was last modified on  July 21, 1986 (version 1) Annotations were last modified on  October 19, 2011 (version 94) Name and origin of the protein Description RecName: Full=Actin; Gene name Name=ACT1 Synonyms=ABY1, END7 OrderedLocusNames=YFL039C Encoded on Name=ACT1; Synonyms=ABY1, END7; OrderedLocusNames=YFL039C Keywords 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL V01288; CAA24597.1; -; Genomic_DNA EMBL V01289; CAA24598.1; ALT_SEQ; Genomic_DNA EMBL V01290; CAA24599.1; -; Genomic_DNA EMBL L00026; AAA34391.1; -; Genomic_DNA EMBL D50617; BAA21512.1; -; Genomic_DNA EMBL AJ389075; CAC00716.1; -; Genomic_DNA EMBL AJ389076; CAC00717.1; -; Genomic_DNA EMBL BK006940; DAA12401.1; -; Genomic_DNA PIR A03005; ATBY; . PIR JS0702; JS0702; . RefSeq NP_116614.1; NM_001179927.1; . PDB 1YAG; X-ray; 1.90 A; A=1-375 PDB 1YVN; X-ray; 2.10 A; A=1-375 PDBsum 1YAG; -; . PDBsum 1YVN; -; . ProteinModelPortal P60010; -; . SMR P60010; 4-375; . DIP DIP-310N; -; . IntAct P60010; 120; . MINT MINT-374866; -; . STRING P60010; -; . SWISS-2DPAGE P60010; -; . PeptideAtlas P60010; -; . EnsemblFungi YFL039C; YFL039C; YFL039C; . GeneID 850504; -; . KEGG sce:YFL039C; -; . NMPDR fig|4932.3.peg.2239; -; . CYGD YFL039c; -; . SGD S000001855; ACT1; . eggNOG fuNOG05202; -; . HOGENOM HBG559892; -; . OMA IKNLMER; -; . OrthoDB EOG4HHS9X; -; . PhylomeDB P60010; -; . NextBio 966206; -; . ArrayExpress P60010; -; . Genevestigator P60010; -; . GermOnline YFL039C; Saccharomyces cerevisiae; . GO GO:0030479; C:actin cortical patch; IDA:SGD; . GO GO:0032432; C:actin filament bundle; IDA:SGD; . GO GO:0000142; C:cellular bud neck contractile ring; IDA:SGD; . GO GO:0031011; C:Ino80 complex; IPI:SGD; . GO GO:0035267; C:NuA4 histone acetyltransferase complex; IPI:SGD; . GO GO:0000812; C:Swr1 complex; IDA:SGD; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0005515; F:protein binding; IPI:UniProtKB; . GO GO:0030476; P:ascospore wall assembly; IDA:SGD; . GO GO:0007119; P:budding cell isotropic bud growth; TAS:SGD; . GO GO:0034599; P:cellular response to oxidative stress; IGI:SGD; . GO GO:0001300; P:chronological cell aging; IMP:SGD; . GO GO:0000916; P:contractile ring contraction involved in cell cycle cytokinesis; IDA:SGD; . GO GO:0006281; P:DNA repair; IDA:SGD; . GO GO:0006897; P:endocytosis; IMP:SGD; . GO GO:0000132; P:establishment of mitotic spindle orientation; TAS:SGD; . GO GO:0006887; P:exocytosis; TAS:SGD; . GO GO:0031505; P:fungal-type cell wall organization; TAS:SGD; . GO GO:0016573; P:histone acetylation; IDA:SGD; . GO GO:0000001; P:mitochondrion inheritance; TAS:SGD; . GO GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:SGD; . GO GO:0000011; P:vacuole inheritance; IMP:SGD; . GO GO:0030050; P:vesicle transport along actin filament; TAS:SGD; . InterPro IPR004000; Actin-like; . InterPro IPR020902; Actin/actin-like_CS; . InterPro IPR004001; Actin_CS; . PANTHER PTHR11937; Actin_like; 1; . Pfam PF00022; Actin; 1; . PRINTS PR00190; ACTIN; . SMART SM00268; ACTIN; 1; . PROSITE PS00406; ACTINS_1; 1; . PROSITE PS00432; ACTINS_2; 1; . PROSITE PS01132; ACTINS_ACT_LIKE; 1; . SWISS-2DPAGE (search AC)