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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P02358




SWISS-2DPAGE:  P02358


P02358


General information about the entry
View entry in simple text format
Entry nameRS6_ECOLI
Primary accession numberP02358
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=30S ribosomal protein S6; Contains: RecName: Full=30S ribosomal protein S6, fully modified isoform; Contains: RecName: Full=30S ribosomal protein S6, non-modified isoform;.
Gene nameName=rpsF
OrderedLocusNames=b4200, JW4158
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[4]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[5]   PROTEIN EXPRESSION
MEDLINE=78212912; PubMed=352533; [NCBI, ExPASy, EBI, Israel, Japan]
Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.
''''''Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates'';'';''
Cell 14(1):179-190(1978)
[6]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.31; Mw=15845
pI=5.15; Mw=15865
pI=5.25; Mw=15845
pI=5.65; Mw=11023  [identification data]

EXPRESSION:
INCREASED IN LEVEL WITH INCREASING GROWTH RATE [5].

MAPPING (identification):
SPOTS 2D-000LQ6, 2D-000LQ7, 2D-000LQA: GEL MATCHING OR AMINO ACID COMPOSITION AND/OR MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3];
SPOT 2D-000LUR: AMINO ACID COMPOSITION AND SEQUENCE TAG (MRHY) [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.30; Mw=16076  [identification data]
pI=5.20; Mw=16000  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [6].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASED014.7; 6TH EDITION.
ECO2DBASEC014.8; 6TH EDITION.
ECO2DBASEC015.3; 6TH EDITION.
UniProtKB/Swiss-ProtP02358; RS6_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRS6_ECOLI
Primary accession numberP02358
Secondary accession number(s) Q2M6A5
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 132)
Name and origin of the protein
DescriptionRecName: Full=30S ribosomal protein S6; Contains: RecName: Full=30S ribosomal protein S6, fully modified isoform; Contains: RecName: Full=30S ribosomal protein S6, non-modified isoform;
Gene nameName=rpsF
OrderedLocusNames=b4200, JW4158
Encoded onName=rpsF; OrderedLocusNames=b4200, JW4158
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX04022; CAA27652.1; ALT_SEQ; Genomic_DNA
EMBLU14003; AAA97096.1; ALT_SEQ; Genomic_DNA
EMBLU00096; AAC77157.5; ALT_SEQ; Genomic_DNA
EMBLAP009048; BAE78201.1; ALT_SEQ; Genomic_DNA
PIRC65231; R3EC6; .
PDB1M5G; Model; -; F=1-100
PDB1P6G; EM; 12.30 A; F=1-135
PDB1P87; EM; 11.50 A; F=1-135
PDB1VS5; X-ray; 3.46 A; F=1-135
PDB1VS7; X-ray; 3.46 A; F=1-135
PDB2AVY; X-ray; 3.46 A; F=1-135
PDB2AW7; X-ray; 3.46 A; F=1-135
PDB2GY9; EM; 15.00 A; F=1-95
PDB2GYB; EM; 15.00 A; F=1-95
PDB2I2P; X-ray; 3.22 A; F=1-135
PDB2I2U; X-ray; 3.22 A; F=1-135
PDB2QAL; X-ray; 3.21 A; F=1-135
PDB2QAN; X-ray; 3.21 A; F=1-135
PDB2QB9; X-ray; 3.54 A; F=1-135
PDB2QBB; X-ray; 3.54 A; F=1-135
PDB2QBD; X-ray; 3.30 A; F=1-135
PDB2QBF; X-ray; 3.30 A; F=1-135
PDB2QBH; X-ray; 4.00 A; F=1-135
PDB2QBJ; X-ray; 4.00 A; F=1-135
PDB2QOU; X-ray; 3.93 A; F=1-135
PDB2QOW; X-ray; 3.93 A; F=1-135
PDB2QOY; X-ray; 3.50 A; F=1-135
PDB2QP0; X-ray; 3.50 A; F=1-135
PDB2VHO; X-ray; 3.74 A; F=1-135
PDB2VHP; X-ray; 3.74 A; F=1-135
PDB2WWL; EM; 5.80 A; F=1-100
PDB2YKR; EM; 9.80 A; F=1-100
PDB2Z4K; X-ray; 4.45 A; F=1-135
PDB2Z4M; X-ray; 4.45 A; F=1-135
PDB3DF1; X-ray; 3.50 A; F=1-135
PDB3DF3; X-ray; 3.50 A; F=1-135
PDB3E1A; EM; -; T=1-135
PDB3E1C; EM; -; T=1-135
PDB3FIH; EM; 6.70 A; F=1-100
PDB3I1M; X-ray; 3.19 A; F=1-135
PDB3I1O; X-ray; 3.19 A; F=1-135
PDB3I1Q; X-ray; 3.81 A; F=1-135
PDB3I1S; X-ray; 3.81 A; F=1-135
PDB3I1Z; X-ray; 3.71 A; F=1-135
PDB3I21; X-ray; 3.71 A; F=1-135
PDB3IZV; EM; -; J=1-135
PDB3IZW; EM; -; J=1-135
PDB3J00; EM; -; F=1-135
PDB3KC4; EM; -; F=1-135
PDB3OAQ; X-ray; 3.25 A; F=1-100
PDB3OAR; X-ray; 3.25 A; F=1-100
PDB3OFA; X-ray; 3.19 A; F=1-100
PDB3OFB; X-ray; 3.19 A; F=1-100
PDB3OFO; X-ray; 3.10 A; F=1-100
PDB3OFP; X-ray; 3.10 A; F=1-100
PDB3OFX; X-ray; 3.29 A; F=1-100
PDB3OFY; X-ray; 3.29 A; F=1-100
PDB3OR9; X-ray; 3.30 A; F=1-135
PDB3ORA; X-ray; 3.30 A; F=1-135
PDB3R8N; X-ray; 3.00 A; F=1-100
PDB3R8O; X-ray; 3.00 A; F=1-100
PDBsum1M5G; -; .
PDBsum1P6G; -; .
PDBsum1P87; -; .
PDBsum1VS5; -; .
PDBsum1VS7; -; .
PDBsum2AVY; -; .
PDBsum2AW7; -; .
PDBsum2GY9; -; .
PDBsum2GYB; -; .
PDBsum2I2P; -; .
PDBsum2I2U; -; .
PDBsum2QAL; -; .
PDBsum2QAN; -; .
PDBsum2QB9; -; .
PDBsum2QBB; -; .
PDBsum2QBD; -; .
PDBsum2QBF; -; .
PDBsum2QBH; -; .
PDBsum2QBJ; -; .
PDBsum2QOU; -; .
PDBsum2QOW; -; .
PDBsum2QOY; -; .
PDBsum2QP0; -; .
PDBsum2VHO; -; .
PDBsum2VHP; -; .
PDBsum2WWL; -; .
PDBsum2YKR; -; .
PDBsum2Z4K; -; .
PDBsum2Z4M; -; .
PDBsum3DF1; -; .
PDBsum3DF3; -; .
PDBsum3E1A; -; .
PDBsum3E1C; -; .
PDBsum3FIH; -; .
PDBsum3I1M; -; .
PDBsum3I1O; -; .
PDBsum3I1Q; -; .
PDBsum3I1S; -; .
PDBsum3I1Z; -; .
PDBsum3I21; -; .
PDBsum3IZV; -; .
PDBsum3IZW; -; .
PDBsum3J00; -; .
PDBsum3KC4; -; .
PDBsum3OAQ; -; .
PDBsum3OAR; -; .
PDBsum3OFA; -; .
PDBsum3OFB; -; .
PDBsum3OFO; -; .
PDBsum3OFP; -; .
PDBsum3OFX; -; .
PDBsum3OFY; -; .
PDBsum3OR9; -; .
PDBsum3ORA; -; .
PDBsum3R8N; -; .
PDBsum3R8O; -; .
ProteinModelPortalP02358; -; .
SMRP02358; 1-129; .
DIPDIP-10782N; -; .
IntActP02358; 56; .
MINTMINT-1279331; -; .
SWISS-2DPAGEP02358; -; .
ECO2DBASEC014.8; 6TH EDITION; .
ECO2DBASEC015.3; 6TH EDITION; .
ECO2DBASED014.7; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004456; EBESCP00000004456; EBESCG00000003635; .
EnsemblBacteriaEBESCT00000017509; EBESCP00000016800; EBESCG00000016565; .
GenomeReviewsAP009048_GR; JW4158; .
GenomeReviewsU00096_GR; b4200; .
KEGGecj:JW4158; -; .
KEGGeco:b4200; -; .
EchoBASEEB0898; -; .
EcoGeneEG10905; rpsF; .
eggNOGCOG0360; -; .
GeneTreeEBGT00050000011094; -; .
HOGENOMHBG747554; -; .
OMAITEASPM; -; .
ProtClustDBPRK00453; -; .
BioCycEcoCyc:EG10905-MONOMER; -; .
GenevestigatorP02358; -; .
GOGO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoliWiki; .
GOGO:0019843; F:rRNA binding; IEA:UniProtKB-KW; .
GOGO:0003735; F:structural constituent of ribosome; IDA:EcoliWiki; .
GOGO:0006412; P:translation; IDA:EcoliWiki; .
HAMAPMF_00360; Ribosomal_S6; 1; -
InterProIPR000529; Ribosomal_S6; .
InterProIPR020815; Ribosomal_S6_CS; .
InterProIPR020814; Ribosomal_S6_plastid/chlpt; .
InterProIPR014717; Transl_elong_EF1B/ribosomal_S6; .
Gene3DG3DSA:3.30.70.60; Transl_elong_EF1B/rib_con; 1; .
PfamPF01250; Ribosomal_S6; 1; .
SUPFAMSSF54995; Ribosomal_S6; 1; .
TIGRFAMsTIGR00166; S6; 1; .
PROSITEPS01048; RIBOSOMAL_S6; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server