World-2DPAGE Home
Home  |  Contact


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AEG4




SWISS-2DPAGE:  P0AEG4


P0AEG4


General information about the entry
View entry in simple text format
Entry nameDSBA_ECOLI
Primary accession numberP0AEG4
Secondary accession number(s) P24991
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Thiol:disulfide interchange protein DsbA; Flags: Precursor;.
Gene nameName=dsbA
Synonyms=dsf, ppfA
OrderedLocusNames=b3860, JW3832
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.34; Mw=22464
pI=5.31; Mw=21973

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.34; Mw=22606  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEE021.2; 6TH EDITION.
ECO2DBASEE021.1; 6TH EDITION.
UniProtKB/Swiss-ProtP0AEG4; DSBA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDSBA_ECOLI
Primary accession numberP0AEG4
Secondary accession number(s) P24991 Q2M8F8 Q46951 Q46952
Sequence was last modified on December 6, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 63)
Name and origin of the protein
DescriptionRecName: Full=Thiol:disulfide interchange protein DsbA; Flags: Precursor;
Gene nameName=dsbA
Synonyms=dsf, ppfA
OrderedLocusNames=b3860, JW3832
Encoded onName=dsbA; Synonyms=dsf, ppfA; OrderedLocusNames=b3860, JW3832
Keywords3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Periplasm; Redox-active center; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX80762; CAA56736.1; -; Genomic_DNA
EMBLM77746; AAA23715.1; -; Genomic_DNA
EMBLX63186; CAA44868.1; -; Genomic_DNA
EMBLL19201; AAB02995.1; -; Genomic_DNA
EMBLU00096; AAC76858.1; -; Genomic_DNA
EMBLAP009048; BAE77448.1; -; Genomic_DNA
EMBLU35817; AAC43519.1; -; Genomic_DNA
EMBLU35818; AAC43520.1; -; Genomic_DNA
EMBLU35819; AAC43521.1; -; Genomic_DNA
EMBLU35820; AAC43522.1; -; Genomic_DNA
EMBLU35821; AAC43523.1; -; Genomic_DNA
EMBLU35822; AAC43524.1; -; Genomic_DNA
EMBLU35823; AAC43525.1; -; Genomic_DNA
EMBLU35824; AAC43526.1; -; Genomic_DNA
EMBLU35825; AAC43527.1; -; Genomic_DNA
EMBLU36828; AAC43528.1; -; Genomic_DNA
EMBLU36829; AAC43529.1; -; Genomic_DNA
EMBLU36830; AAC43530.1; -; Genomic_DNA
EMBLU36831; AAC43531.1; -; Genomic_DNA
EMBLU36832; AAC43532.1; -; Genomic_DNA
EMBLU36833; AAC43533.1; -; Genomic_DNA
EMBLU36834; AAC43534.1; -; Genomic_DNA
EMBLU36835; AAC43535.1; -; Genomic_DNA
EMBLU35662; AAA82614.1; -; Genomic_DNA
PIRA39292; A39292; .
RefSeqNP_418297.1; NC_000913.2; .
PDB1A23; NMR; -; A=20-208
PDB1A24; NMR; -; A=20-208
PDB1A2J; X-ray; 2.00 A; A=20-208
PDB1A2L; X-ray; 2.70 A; A/B=20-208
PDB1A2M; X-ray; 2.70 A; A/B=20-208
PDB1AC1; X-ray; 2.00 A; A/B=20-208
PDB1ACV; X-ray; 1.90 A; A/B=20-208
PDB1BQ7; X-ray; 2.80 A; A/B/C/D/E/F=20-208
PDB1DSB; X-ray; 2.00 A; A/B=20-208
PDB1FVJ; X-ray; 2.06 A; A/B=20-208
PDB1FVK; X-ray; 1.70 A; A/B=20-208
PDB1TI1; X-ray; 2.60 A; A=20-208
PDB1U3A; X-ray; 2.00 A; A/B/D/E=20-208
PDB1UN2; X-ray; 2.40 A; A=20-208
PDB2B3S; X-ray; 1.96 A; A/B=20-208
PDB2B6M; X-ray; 2.65 A; A/B=20-208
PDB2HI7; X-ray; 3.70 A; A=20-208
PDB2ZUP; X-ray; 3.70 A; A=20-208
PDB3E9J; X-ray; 3.70 A; B/E=20-208
PDBsum1A23; -; .
PDBsum1A24; -; .
PDBsum1A2J; -; .
PDBsum1A2L; -; .
PDBsum1A2M; -; .
PDBsum1AC1; -; .
PDBsum1ACV; -; .
PDBsum1BQ7; -; .
PDBsum1DSB; -; .
PDBsum1FVJ; -; .
PDBsum1FVK; -; .
PDBsum1TI1; -; .
PDBsum1U3A; -; .
PDBsum1UN2; -; .
PDBsum2B3S; -; .
PDBsum2B6M; -; .
PDBsum2HI7; -; .
PDBsum2ZUP; -; .
PDBsum3E9J; -; .
ProteinModelPortalP0AEG4; -; .
SMRP0AEG4; 20-207; .
DIPDIP-35886N; -; .
IntActP0AEG4; 19; .
MINTMINT-1247943; -; .
SWISS-2DPAGEP0AEG4; -; .
ECO2DBASEE021.1; 6TH EDITION; .
ECO2DBASEE021.2; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000414; EBESCP00000000414; EBESCG00000000343; .
EnsemblBacteriaEBESCT00000000415; EBESCP00000000415; EBESCG00000000343; .
EnsemblBacteriaEBESCT00000017977; EBESCP00000017268; EBESCG00000017033; .
GeneID948353; -; .
GenomeReviewsAP009048_GR; JW3832; .
GenomeReviewsU00096_GR; b3860; .
KEGGecj:JW3832; -; .
KEGGeco:b3860; -; .
EchoBASEEB1274; -; .
EcoGeneEG11297; dsbA; .
eggNOGCOG0526; -; .
GeneTreeEBGT00050000010220; -; .
HOGENOMHBG680642; -; .
OMAMFEAVQK; -; .
ProtClustDBPRK10954; -; .
BioCycEcoCyc:DISULFOXRED-MONOMER; -; .
BioCycMetaCyc:DISULFOXRED-MONOMER; -; .
GenevestigatorP0AEG4; -; .
GOGO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; .
GOGO:0045454; P:cell redox homeostasis; IEA:InterPro; .
InterProIPR001853; DSBA-like_thioredoxin_dom; .
InterProIPR023205; Thiol:disulphide_interchange; .
InterProIPR012336; Thioredoxin-like_fold; .
InterProIPR017937; Thioredoxin_CS; .
Gene3DG3DSA:3.40.30.10; Thioredoxin_fold; 2; .
PfamPF01323; DSBA; 1; .
PIRSFPIRSF001488; Tdi_protein; 1; .
SUPFAMSSF52833; Thiordxn-like_fd; 1; .
PROSITEPS00194; THIOREDOXIN_1; 1; .
PROSITEPS51352; THIOREDOXIN_2; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server